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Home >> Antibodies >> GATA-3 (Center) (Breast and Urothelial Marker)

GATA-3 (Center) (Breast and Urothelial Marker)

Mouse Monoclonal Antibody [Clone GATA3/6664]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Formalin-fixed, paraffin-embedded human lobular breast carcinoma stained with GATA-3 Mouse Monoclonal Antibody (GATA3/6664) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

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SDS-PAGE Analysis Purified GATA-3 Mouse Monoclonal Antibody (GATA3/6664). Confirmation of Purity and Integrity of Antibody.

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Formalin-fixed, paraffin-embedded human lobular breast carcinoma stained with GATA-3 Mouse Monoclonal Antibody (GATA3/6664).

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Formalin-fixed, paraffin-embedded human bladder stained with GATA-3 Mouse Monoclonal Antibody (GATA3/6664).

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IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using GATA3/6664 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Product Details

Synonyms

GATA binding protein-3; GATA-binding factor 3; HDR; HDRS; Transacting T-cell-specific transcription factor GATA-3

Positive Control

Human breast or urothelial carcinoma tissue.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human GATA-3 protein

Cellular Localization

Nucleus.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

50kDa

Specificity & Comments

GATA-3 (GATA binding protein 3) is a member of the GATA family of transcription factors. This 50kDa nuclear protein regulates the development and subsequent maintenance of a variety of human tissues, including hematopoietic cells, skin, kidney, mammary gland, and the central nervous system. Among several other roles, GATA-3 involved in luminal cell differentiation in the mammary gland and appears to control a set of genes involved in the differentiation and proliferation of breast cancer. The expression of GATA-3 is associated with the expression of estrogen receptor-alpha (ER) in breast cancer. GATA-3 has been shown to be a novel marker for bladder cancer. The study demonstrated that GATA-3 stained 67% of urothelial Carcinomas, but none of prostate or renal carcinomas.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Higgins JP, et al. Am J Surg Pathol. 2007; 31:673-680.
  2. Liu H, et al. Am J Clin Pathol. 2012; 138:57-64.

Bioinformatics

  •  Entrez Gene ID: 2625
  •  Gene Symbol: GATA3
  •  SwissProt: P23771
  •  Chromosome Location: 10p14
  •  Unigene: 524134