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GATA-3 (Center) (Breast and Urothelial Marker) Antibody [MSVA-450M]

In Stock
Catalog Number Formulation Size Price
2625-MSM27-P0
Purified Ab with BSA and Azide
20ug
$434.50
2625-MSM27-P1
Purified Ab with BSA and Azide
100ug
$984.50
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
GATA3 Antibody in Numerous Tissues (IHC)

GATA3 Antibody in Numerous Tissues (IHC)

Trans-acting T-cell-specific transcription factor GATA-3 Mouse Monoclonal Antibody (MSVA-450M) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1:50-1:100
IHC Protocol
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:80 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Summary

GATA-3 (GATA binding protein 3) is a member of the GATA family of transcription factors. This 50kDa nuclear protein regulates the development and subsequent maintenance of a variety of human tissues, including hematopoietic cells, skin, kidney, mammary gland, and the central nervous system. Among several other roles, GATA-3 involved in luminal cell differentiation in the mammary gland and appears to control a set of genes involved in the differentiation and proliferation of breast cancer. The expression of GATA-3 is associated with the expression of estrogen receptor-alpha (ER) in breast cancer. GATA-3 has been shown to be a novel marker for bladder cancer. The study demonstrated that GATA-3 stained 67% of urothelial Carcinomas, but none of prostate or renal carcinomas.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular, Immunology, Cytokine Signaling, Nuclear Marker, Signal Transduction, Transcription Factors
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Nucleus
Gene Name
GATA3
Positive Control
Kidney: A moderate to strong nuclear staining reaction should be seen in a fraction of collecting duct cells and podocytes in glomeruli. ; Tonsil: The vast majority of T helper cells (Th2) in the T-zones should show a weak to moderate nuclear staining.
Immunogen
Recombinant full-length human GATA-3 protein
Alternate Names
Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA binding protein-3; GATA-binding factor 3; HDR; HDRS; Transacting T-cell-specific transcription factor GATA-3

Database Links

Entrez Gene ID
2625
SwissProt
P23771

Additional Information

Clone
MSVA-450M
Chromosome Location
10p14
Mol. Weight of Antigen
~50kDa

Related Antibodies

View All GATA3 Antibodies

Functions

  • Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses. Positively regulates ASB2 expression (By similarity).

Key References

  • Higgins JP, et al. Am J Surg Pathol. 2007; 31:673-680.
  • Liu H, et al. Am J Clin Pathol. 2012; 138:57-64.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

Ab purified from Bioreactor Concentrate by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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