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Home >> Antibodies >> Ep-CAM / CD326 (Rat)

Ep-CAM / CD326 (Rat)

Mouse Monoclonal Antibody [Clone SPM534]

Catalog #

Size

0.1 ml
0.5 ml

Price (USD)

199.00
429

Formulation

Bioreactor Concentrate
Bioreactor Concentrate

Catalog No

Size

0.1 ml
0.5 ml

Formulation

Bioreactor Concentrate
Bioreactor Concentrate

Price (USD)

199.00
429
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Western Blot Analysis of rat Colon lysate probed with Ep-CAM Monoclonal Antibody (SPM534).

Product Details

Synonyms

Adenocarcinoma-associated Antigen; Cell Surface Glycoprotein Trop-1; EGP2; EGP314; EGP40; Epithelial Cell Adhesion Molecule; Epithelial Glycoprotein 314; ESA; KSA; TACD1; TACSTD1; TROP1; Tumor-associated Calcium Signal Transducer 1, ECS-1, Epidermal Surface Antigen 1, ESA1, FLOT2, Flotillin-2, M17S1 (Membrane Component, Chromosome 17, Surface Marker-1), REG-1, Reggie-1, Reggie-2

Positive Control

Rat small intestine or colon.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IP, IHC         More Details

Flow Cytometry (5-10ul/million cells)
Immunofluorescence (1:50-1:100)
Western Blot (1:100-1:200)
Immunoprecipitation (5-10ul/500ug protein lysate)
Immunohistochemistry (Frozen & formalin-fixed) (1:100-1:200 for 30 min at RT)(Formalin/paraffin tissues REQUIRE digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37C)Optimal dilution for a specific application should be determined.

Immunogen

Rat small intestine cells

Cellular Localization

Cell surface

Species Reactivity

Rat. Does not react with Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

40-43kDa

Specificity & Comments

Epitope of GZ-1 and GZ-20 monoclonal antibodies are different. EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas.

Related Products



Bioreactor Concentrate with 0.05% Azide. Contact us if you require it in a different format.

Key References

  1. Litvinov SV et. al. J Cell Biol, 1994, 125:437-46.
  2. Tsubura A et. al. J Cut Pathol, 1992, 19:73-9.

Bioinformatics

  •  Entrez Gene ID: 171577
  •  SwissProt: O55159
NBT00381