Ep-CAM / CD326 (Rat) [SPM534]

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Summary

Epitope of GZ-1 and GZ-20 monoclonal antibodies are different. EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas.

Product Properties & Targets

Host
Species Reactivity
Rat
Isotype
Gene Name
EPCAM
Mol. Weight of Antigen
40-43kDa
Immunogen
Rat small intestine cells

Database Links

Entrez Gene ID
Unigene
SwissProt

Additional Information

Clone
SPM534

Known Applications & Suggested Dilutions

  • Flow Cytometry (5-10ul/million cells)
  • Immunofluorescence (1:50-1:100)
  • Western Blot (1:100-1:200)
  • Immunoprecipitation (5-10ul/500ug protein lysate)
  • Immunohistochemistry (Frozen & formalin-fixed) (1:100-1:200 for 30 min at RT)(Formalin/paraffin tissues REQUIRE digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37C)Optimal dilution for a specific application should be determined.

Key References

  • Litvinov SV et. al. J Cell Biol, 1994, 125:437-46.
  • Tsubura A et. al. J Cut Pathol, 1992, 19:73-9.

Storage & Stability

Store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

Bioreactor Concentrate with 0.05% Azide. Contact us if you require it in a different format.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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