Home >> Antibodies >> Desmin (Muscle Cell Marker)

Desmin (Muscle Cell Marker)

Mouse Monoclonal Antibody [Clone DES/4526]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human skeletal muscle stained with Desmin Mouse Monoclonal Antibody (DES/4526) at 2ug/ml. Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human uterus stained with Desmin Mouse Monoclonal Antibody (DES/4526). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human small intestine stained with Desmin Mouse Monoclonal Antibody (DES/4526). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human small intestine stained with Desmin Mouse Monoclonal Antibody (DES/4526). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human skeletal muscle stained with Desmin Mouse Monoclonal Antibody (DES/4526) at 2ug/ml. Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human uterus stained with Desmin Mouse Monoclonal Antibody (DES/4526). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human small intestine stained with Desmin Mouse Monoclonal Antibody (DES/4526). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human small intestine stained with Desmin Mouse Monoclonal Antibody (DES/4526). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Product Details

Synonyms

CMD1I; CSM1; CSM2; DES; Intermediate filament protein

Positive Control

SJRH30 cells. Human uterus, small intestine or leiomyosarcoma.

Known Applications & Suggested Dilutions

IHC-P More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human desmin protein

Cellular Localization

Cytoplasm.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

52kDa

Specificity & Comments

Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. IFs are constructed from two-chain -helical coiled-coil molecules arranged on an imperfect helical lattice, and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. Vimentin is an IF general marker of cells originating in the mesenchyme. Vimentin and Desmin, a related class III IF, are both expressed during skeletal muscle development. Desmin, a 469 amino acid protein found near the Z line in sarcomeres, is expressed more frequently in adult differentiated state tissues. Anti-desmin detects cells of normal smooth, skeletal, and cardiac muscles.Antibody reacts with leiomyomas, leiomyosarcoma, rhabdomyomas, rhabdomyosarcoma, and perivascular cells of glomus tumors of the skin.