Formalin-fixed, paraffin-embedded human skeletal muscle stained with Desmin Mouse Monoclonal Antibody (DES/4526) at 2ug/ml. Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human uterus stained with Desmin Mouse Monoclonal Antibody (DES/4526). HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human small intestine stained with Desmin Mouse Monoclonal Antibody (DES/4526). HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

Printable Datasheet

Desmin (Muscle Cell Marker) [DES/4526]

Name: Desmin (Muscle Cell Marker)
SKU: 1674-MSM6
Availability: InStock

Monoclonals Mouse Monoclonals Primary Antibodies

Summary

Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. IFs are constructed from two-chain 伪-helical coiled-coil molecules arranged on an imperfect helical lattice, and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. Vimentin is an IF general marker of cells originating in the mesenchyme. Vimentin and Desmin, a related class III IF, are both expressed during skeletal muscle development. Desmin, a 469 amino acid protein found near the Z line in sarcomeres, is expressed more frequently in adult differentiated state tissues. Anti-desmin detects cells of normal smooth, skeletal, and cardiac muscles.Antibody reacts with leiomyomas, leiomyosarcoma, rhabdomyomas, rhabdomyosarcoma, and perivascular cells of glomus tumors of the skin.

Product Properties & Targets

Host

Mouse

Species Reactivity

Human

Isotype

IgG1

Cellular Localization

Cell membrane, Cytoplasm, Myofibril, Nucleus, Sarcolemma, Sarcomere, Z line

Light Chain

kappa

Gene Name

DES

Positive Control

SJRH30 cells. Human uterus, small intestine or leiomyosarcoma.

Immunogen

Recombinant full-length human desmin protein

Alternate Names

Desmin, CMD1I; CSM1; CSM2; DES; Intermediate filament protein

Database Links

Entrez Gene ID

1674

Unigene

594952

SwissProt

P17661

Additional Information

Clone

DES/4526

Human Gene Symbol

DES

Chromosome Location

2q35

Mol. Weight of Antigen

52kDa

Functions

Muscle-specific type III intermediate filament essential for proper muscular structure and function. Plays a crucial role in maintaining the structure of sarcomeres, inter-connecting the Z-disks and forming the myofibrils, linking them not only to the sarcolemmal cytoskeleton, but also to the nucleus and mitochondria, thus providing strength for the muscle fiber during activity (PubMed:25358400). In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures (PubMed:24200904, PubMed:25394388, PubMed:26724190). May act as a sarcomeric microtubule-anchoring protein: specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction. Contributes to the transcriptional regulation of the NKX2-5 gene in cardiac progenitor cells during a short period of cardiomyogenesis and in cardiac side population stem cells in the adult. Plays a role in maintaining an optimal conformation of nebulette (NEB) on heart muscle sarcomeres to bind and recruit cardiac alpha-actin (By similarity).

Known Applications & Suggested Dilutions

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

Li, Z.L., et al. 1989. Gene 78: 243-254.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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