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Home >> Antibodies >> Cytokeratin, Multi (Epithelial Marker)

Cytokeratin, Multi (Epithelial Marker)

Mouse Monoclonal Antibody [Clone C11]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Multi Cytokeratin Mouse Monoclonal Antibody (C11).

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Formalin-fixed, paraffin-embedded Rat Oviduct stained with Multi Cytokeratin Mouse Monoclonal Antibody (C11).

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SDS-PAGE Analysis Purified Multi Cytokeratin Mouse Monoclonal Antibody (C11).

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Immunofluorescence Analysis of HeLa cells labeling Multi Cytokeratin with Multi Cytokeratin Mouse Monoclonal Antibody (C11) conjugated with CF640R(Green). The nuclear counterstain is DAPI (Blue)

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Flow Cytometric Analysis of Human HeLa cells using Multi Cytokeratin Mouse Monoclonal Antibody (C11) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

Product Details

Positive Control

HeLa or A431 cells. Skin or colon carcinoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-4ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Keratin-enriched preparation from cultured human A431

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Cow. Rat. Mouse. Guinea Pig. Frog. Goat. Marmoset. Pig.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

Multiple

Specificity & Comments

This MAb recognizes cytokeratin 4, 5, 6, 8, 10, 13, and 18. This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Folia Biol., 35(6), 373-382, (1989)
  2. Vojtek et al. Neoplasma 37(3), 333-342 (1990).
  3. Kovaik et al. J. Tumor Marker Oncol. 5, 219 (1990).
  4. Kovaik et al. Int.J.Cancer, Suppl. 3: 50-55, (1988).
  5. Lane EB; Alexander CM. Seminars in Cancer Biology, 1990, 1:165-79.

Bioinformatics

NBT00393