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Home >> Antibodies >> Cytokeratin 5/6/18

Cytokeratin 5/6/18

Mouse Monoclonal Antibody [Clone SPM267]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin, 5/6/18 Mouse Monoclonal Antibody (SPM267).

img

SDS-PAGE Analysis Purified Cytokeratin, 5/6/18 Mouse Monoclonal Antibody (SPM267). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

CK5; Keratin-5; KRT5; Cytoskeletal 6,; KRT6, CK18; CYK18; K18; Keratin-18; KRT18

Positive Control

HeLa, HepG2 or PC12 cells. Tonsil or Skin.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Detergent-insoluble fraction of psoriatic human epidermis

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Cow. Rat.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

45-58kDa

Specificity & Comments

It recognizes polypeptides of 58kDa, 56kDa, and 45kDa, identified as cytokeratin 5, 6, and 18 respectively. It shows no reaction with keratin 1, 8, or 19. This MAb shows a broad pattern of reactivity with epithelial tissues, from simple glandular epithelia to stratified squamous epithelia. Epithelial cells are labeled whether they are ectodermal, mesodermal, or endodermal in origin. This MAb is useful in detecting cells of epithelial origin in mixed tumors, in effusions, in bone marrow samples, or in tissue culture cells.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Lane EB, et. al. Annals of the New York Academy of Sciences, 1985, 455:241-58.

Bioinformatics

  •  Entrez Gene ID: Multiple
  •  Gene Symbol: Multiple
  •  SwissProt: Multiple
  •  Chromosome Location: Multiple
  •  Unigene: Multiple
NBT02036