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Home >> Antibodies >> Cytokeratin 4 (KRT4)

Cytokeratin 4 (KRT4)

Mouse Monoclonal Antibody [Clone KRT4/2804]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin 4 Mouse Monoclonal Antibody (KRT4/2804).

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Flow Cytometric Analysis of HeLa cells using Cytokeratin 4 Mouse Monoclonal Antibody (KRT4/2804)followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

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Immunofluorescence Analysis of A549 cells labeling KRT4 with Cytokeratin 4 Mouse Monoclonal Antibody (KRT4/2804)followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using Cytokeratin 4 Mouse Monoclonal Antibody (KRT4/2804).

Product Details

Synonyms

CK4; CYK4; Cytokeratin 4; Keratin 4; Keratin type II cytoskeletal 4; KRT4

Positive Control

HeLa, A549 or A431 cells. Skin, Cervix or Esophagus.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa 181-292) of human KRT4 protein (exact sequence is proprietary)

Cellular Localization

Cytoplasmic and Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

67kDa

Specificity & Comments

Cytokeratin 4 (KRT4) is an intermediate filament protein associated with Cytokeratin 13 (KRT13). It is expressed in suprabasal cells of non-keratinized stratified squamous epithelium of esophagus, cornea, anus, larynx, pharynx and tongue. Decreased expression of KRT4 is associated with head and neck squamous carcinoma. It is helpful in differentiation of squamous cell carcinoma of esophagus origin from that of thyroid origin.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Opitz Og, et al. J Biol Chem. 1998 Sep 11;273(37):23912-21.

Bioinformatics

  •  Entrez Gene ID: 3851
  •  Gene Symbol: KRT4
  •  SwissProt: P19013
  •  Chromosome Location: 12q13.13
  •  Unigene: 654610, 706527
NBT02804