Home >> Antibodies >> Cytokeratin 19 (Pancreatic Stem Cell Marker)

Cytokeratin 19 (Pancreatic Stem Cell Marker)

Mouse Monoclonal Antibody [Clone KRT19/800]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human pancreas stained with Cytokeratin 19 Mouse Monoclonal Antibody (KRT19/800).

Formalin-paraffin human endometrial carcinoma stained with Cytokeratin 19 Mouse Monoclonal Antibody (KRT19/800).

Western blot analysis of human lung tissue lysate using Cytokeratin 19 Mouse Monoclonal Antibody (KRT19/800).

Immunofluorescence Analysis of MeOH-fixed MCF-7 cells labeling CK19. Cytokeratin 19 Mouse Monoclonal Antibody (KRT19/800) followed by goat anti-mouse IgG-CF488 (cyan). Nuclear counterstain is RedDot (red).

Formalin-fixed, paraffin-embedded rat stomach stained with Cytokeratin 19 Mouse Monoclonal Antibody (KRT19/800).

Formalin-fixed, paraffin-embedded rat colon stained with Cytokeratin 19 Mouse Monoclonal Antibody (KRT19/800).

Formalin-fixed, paraffin-embedded human cervical carcinoma stained with Cytokeratin 19 Mouse Monoclonal Antibody (KRT19/800).

Formalin-fixed, paraffin-embedded rat lung stained with Cytokeratin 19 Mouse Monoclonal Antibody (KRT19/800).

Formalin-fixed, paraffin-embedded human pancreas stained with Cytokeratin 19 Mouse Monoclonal Antibody (KRT19/800).

Formalin-paraffin human endometrial carcinoma stained with Cytokeratin 19 Mouse Monoclonal Antibody (KRT19/800).

Western blot analysis of human lung tissue lysate using Cytokeratin 19 Mouse Monoclonal Antibody (KRT19/800).

Immunofluorescence Analysis of MeOH-fixed MCF-7 cells labeling CK19. Cytokeratin 19 Mouse Monoclonal Antibody (KRT19/800) followed by goat anti-mouse IgG-CF488 (cyan). Nuclear counterstain is RedDot (red).

Formalin-fixed, paraffin-embedded rat stomach stained with Cytokeratin 19 Mouse Monoclonal Antibody (KRT19/800).

Formalin-fixed, paraffin-embedded rat colon stained with Cytokeratin 19 Mouse Monoclonal Antibody (KRT19/800).

Formalin-fixed, paraffin-embedded human cervical carcinoma stained with Cytokeratin 19 Mouse Monoclonal Antibody (KRT19/800).

Formalin-fixed, paraffin-embedded rat lung stained with Cytokeratin 19 Mouse Monoclonal Antibody (KRT19/800).

Product Details

Synonyms

k19; k1cs; Keratin 19 Keratin Type i 40kD; KRT19

Positive Control

MCF-7 or HeLa cells. Breast cancer tissues.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P More Details

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human KRT19 protein.

Cellular Localization

Cytoplasm

Species Reactivity

Human, Mouse and Rat.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

40kDa

Specificity & Comments

Recognizes a protein of 40kDa, identified as cytokeratin-19 (CK19), which is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.