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Home >> Antibodies >> Cytokeratin 10 (Suprabasal Epithelial Marker)

Cytokeratin 10 (Suprabasal Epithelial Marker)

Mouse Monoclonal Antibody [Clone KRT10/844]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
img

Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin 10 Mouse Monoclonal Antibody (KRT10/844).

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Western Blot Analysis of human Thymus tissue lysate using Cytokeratin 10 Mouse Monoclonal Antibody (KRT10/844).

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SDS-PAGE Analysis Purified Cytokeratin 10 Monoclonal Antibody (KRT10/844). Confirmation of Integrity and Purity of Antibody

Product Details

Synonyms

BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10

Positive Control

A431, HeLa, MCF7 cells. Esophagus or tonsil.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human KRT10 protein

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Mouse.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

56.5kDa

Specificity & Comments

This MAb recognizes a protein of 56.5kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. van der Velden, L.A., et al. 1993. Cytokeratin expression in normal and (pre) malignant head and neck epithelia: an overview. Head Neck 15: 133-146.

Bioinformatics

  •  Entrez Gene ID: 3858
  •  Gene Symbol: KRT10
  •  SwissProt: P13645
  •  Chromosome Location: 17q21.2
  •  Unigene: 99936
NBT00844