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Home >> Antibodies >> Clusterin / Apolipoprotein J (APO-J)

Clusterin / Apolipoprotein J (APO-J)

Mouse Monoclonal Antibody [Clone CLU/4733]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Formalin-fixed, paraffin-embedded human adrenal gland stained with Clusterin / APOJ Mouse Monoclonal Antibody (CLU/4733).

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SDS-PAGE Analysis Purified Clusterin / APOJ Mouse Monoclonal Antibody (CLU/4733). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using Monospecific to Clusterin / APOJ Mouse Monoclonal Antibody (CLU/4733).

Product Details

Synonyms

Aging associated protein 4; Apolipoprotein J; CLU1; CLU2; Clusterin alpha / beta chain; Complement associated protein SP40; Complement cytolysis inhibitor a chain; NA1/NA2; D14Ucla3; Dimeric acid glycoprotein; GP80; Ku70-binding protein 1 (KUB1); Sulfated glycoprotein 2 (SGP2), Testosterone repressed prostate message 2 (TPRM2B)

Positive Control

Human pancreas, tonsil or cervix.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa150-300) of human CLU protein (exact sequence is proprietary)

Cellular Localization

Cytoplasm and Nucleus.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

70kDa (precursor); 36-39kDa (α); 34-36kDa (β)

Specificity & Comments

Clusterin, also designated complement lysis inhibitor (CLI), apolipoprotein J (APOJ), sulfated glycoprotein 2 (SGP2), SP40 and testosterone-repressed prostate message 2 (TRPM2), is a secretory, heterodimeric glycoprotein that influences immune regulation, cell adhesion, transformation, lipid transportation, tissue remodeling, membrane recycling and cell-cell interactions. Clusterin is synthesized as a 449 amino acid polypeptide that is post-translationally cleaved at an internal bond between Arg 227 and Ser 228. Two subunits, α and β, are associated through disulfide bonds. The β subunit (also called ApoJα) corresponds to residues 23-227. The α subunit (also called ApoJβ) corresponds to residues 228-449. Overexpression of Clusterin appears to be more common in late stages of mammary tumor progression. Clusterin markedly influences β-Amyloid structure and neuritic toxicity in vivo and may influence Alzheimer s pathogenesis.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Fu-Jun, L., et al. 2012. Oncol. Lett. 4: 1097-1103.

Bioinformatics

  •  Entrez Gene ID: 1191
  •  Gene Symbol: CLU
  •  SwissProt: P10909
  •  Chromosome Location: 8p21.1
  •  Unigene: 436657