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Home >> Antibodies >> Clusterin / Apolipoprotein J (APO-J)

Clusterin / Apolipoprotein J (APO-J)

Mouse Monoclonal Antibody [Clone CLU/4731]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Western Blot Analysis of human tissue lysate using Clusterin / APOJ Mouse Monoclonal Antibody (CLU/4731).

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Formalin-fixed, paraffin-embedded human adrenal gland stained with Clusterin / APOJ Mouse Monoclonal Antibody (CLU/4731).

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SDS-PAGE Analysis Purified Clusterin / APOJ Mouse Monoclonal Antibody (CLU/4731). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using Clusterin / APOJ Mouse Monoclonal Antibody (CLU/4731).

Product Details

Synonyms

Aging associated protein 4; Apolipoprotein J; CLU1; CLU2; Clusterin alpha / beta chain; Complement associated protein SP40; Complement cytolysis inhibitor a chain; NA1/NA2; D14Ucla3; Dimeric acid glycoprotein; GP80; Ku70-binding protein 1 (KUB1); Sulfated glycoprotein 2 (SGP2), Testosterone repressed prostate message 2 (TPRM2B)

Positive Control

Human pancreas, tonsil or cervix.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (2-4ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa150-300) of human CLU protein (exact sequence is proprietary)

Cellular Localization

Secreted. Can retrotranslocate from secretory compartments to cytosol upon cellular stress and Nucleus. Cytoplasm. Mitochondrion membrane. Cytoplasm, cytosol. Microsome. Endoplasmic reticulum. Cytopla

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

70kDa (precursor); 36-39kDa (α); 34-36kDa (β)

Specificity & Comments

Clusterin, also designated complement lysis inhibitor (CLI), apolipoprotein J (APOJ), sulfated glycoprotein 2 (SGP2), SP40 and testosterone-repressed prostate message 2 (TRPM2), is a secretory, heterodimeric glycoprotein that influences immune regulation, cell adhesion, transformation, lipid transportation, tissue remodeling, membrane recycling and cell-cell interactions. Clusterin is synthesized as a 449 amino acid polypeptide that is post-translationally cleaved at an internal bond between Arg 227 and Ser 228. Two subunits, α and β, are associated through disulfide bonds. The β subunit (also called ApoJα) corresponds to residues 23-227. The α subunit (also called ApoJβ) corresponds to residues 228-449. Overexpression of Clusterin appears to be more common in late stages of mammary tumor progression. Clusterin markedly influences β-Amyloid structure and neuritic toxicity in vivo and may influence Alzheimer s pathogenesis.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Fu-Jun, L., et al. 2012. Oncol. Lett. 4: 1097-1103.

Bioinformatics

  •  Entrez Gene ID: 1191
  •  Gene Symbol: CLU
  •  SwissProt: P10909
  •  Chromosome Location: 8p21.1
  •  Unigene: 436657