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Home >> Antibodies >> CD8A (Cytotoxic / Suppressor T-Cell Marker)

CD8A (Cytotoxic / Suppressor T-Cell Marker)

Mouse Monoclonal Antibody [Clone RIV11]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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SDS-PAGE Analysis Purified CD8A Mouse Monoclonal Antibody (RIV11). Confirmation of Integrity and Purity of Antibody.

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Immunofluorescence staining of human PBMC cells. CD8A Mouse Monoclonal Antibody (RIV11) followed by goat anti-mouse IgG-CF488 (green).

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Flow Cytometric Analysis of human PBMC cells. CD8A Mouse Monoclonal Antibody (RIV11) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

Product Details

Synonyms

CD8 antigen, alpha polypeptide (p32), T8/Leu-2 T-lymphocyte differentiation antigen, Ly3, LYT3, MAL, T-cell surface glycoprotein CD8 alpha chain

Positive Control

HuT78 or hPBL. Human tonsil.

Known Applications & Suggested Dilutions

Flow Cyt, IF         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human CD8A protein

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

32kDa

Specificity & Comments

Cluster of Differentiation 8 (CD8) is a disulphide-linked heterodimer consisting of the unrelated and subunits. Each subunit is a glycoprotein composed of a single extracellular Ig-like domain, a polypeptide linker, a transmembrane part and a short cytoplasmic tail. On T cells, CD8 is the coreceptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen Major Histocompatibility Complex (MHC). Specifically, the Ig-like domain of CD8 interacts with the 3-domain of the MHC class I molecule. CD8 ensures specificity of the TCR antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and the chain recruits the tyrosine kinase Lck, which is essential for T cell activation.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Knapp W. et. al. Leukocyte Typing IV, p342-343, Oxford University Press, 1989
  2. Parnes JR, CD4 and CD8 in T cell lineage commitment: alterations induced by expression of a CD8/CD4 chimeric transgene. Semin Immunol 1994, 6:221-229.

Bioinformatics

  •  Entrez Gene ID: 925
  •  Gene Symbol: CD8A
  •  SwissProt: P01732
  •  Chromosome Location: 2p11.2
  •  Unigene: 85258