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CD63 (Late Endosomes Marker)

Mouse Monoclonal Antibody [Clone LAMP3/4949]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Product Details

Synonyms

gp55; granulophysin; Lysosomal-associated membrane protein 3; Mast cell antigen AD1Melanoma-associated antigen MLA1; Melanoma-associated antigen ME491; NGA; Ocular melanoma-associated antigen; Tetraspanin-30; TSPAN30

Positive Control

HL60, THP-1 or NIH/3T3 cells. Human spleen, melanoma or lymphoma.

Known Applications & Suggested Dilutions

Functional studies, IP, Flow Cyt, IF, IHC-P More Details

ELISA
Functional studies
Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate))
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °
C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Full-length human CD63 protein

Cellular Localization

Cell surface. Cytoplasm.

Species Reactivity

Human. Porcine.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

26kDa (core protein); 30-60kDa (glycosylated)

Specificity & Comments

CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.