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CD45RA / LCA (B-Cell Marker)

Recombinant Mouse Monoclonal Antibody [Clone rPTPRC/7284]

Printable

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Bioinformatics

Formalin-fixed, paraffin-embedded human tonsil stained with CD45RA Recombinant Mouse Monoclonal Antibody (rPTPRC/7284). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human tonsil stained with CD45RA Recombinant Mouse Monoclonal Antibody (rPTPRC/7284). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human tonsil stained with CD45RA Recombinant Mouse Monoclonal Antibody (rPTPRC/7284). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human tonsil stained with CD45RA Recombinant Mouse Monoclonal Antibody (rPTPRC/7284). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Product Details

Synonyms

B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein

Positive Control

Jurkat, Ramos, U-698, or GA-10 cells. Human tonsil or lymph node.

Known Applications & Suggested Dilutions

IHC-P More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human PTPRC protein

Cellular Localization

Cell surface. Cytoplasm.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

180-220kDa

Specificity & Comments

CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.