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Home >> Antibodies >> CD34 (Hematopoietic Stem Cell & Endothelial Marker)

CD34 (Hematopoietic Stem Cell & Endothelial Marker)

Mouse Monoclonal Antibody [Clone SPM610]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Angiosarcoma stained with CD34 Monoclonal Antibody (SPM610)

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SDS-PAGE Analysis Purified CD34 Monoclonal Antibody (SPM610). Confirmation of Integrity and Purity of Antibody.

Product Details

Synonyms

Hematopoietic Progenitor Cell Antigen, HPCA1, Mucosialin

Positive Control

KG-1 cells. Human tonsil or angiosarcoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 min at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human HPCA1 protein

Cellular Localization

Cell surface

Species Reactivity

Human. Rat.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

90-110kDa

Specificity & Comments

This antibody recognizes a carbohydrate epitope on a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34 (VI international workshop on human differentiation antigens). Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels 85% of angiosarcoma and Kaposi s sarcoma, but shows low specificity.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Sato T et al. Blood 94:2548-2554 (1999)
  2. Felshow DM et al. Blood 97:3768-3775 (2001)

Bioinformatics

  •  Entrez Gene ID: 947
  •  Gene Symbol: CD34
  •  SwissProt: P28906
  •  Chromosome Location: 1q32.2
  •  Unigene: 374990
NBT01171