2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> CD19 (B-Lymphocyte Marker)

CD19 (B-Lymphocyte Marker)

Mouse Monoclonal Antibody [Clone CD19/3116]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Tonsil stained with CD19 Monospecific Mouse Monoclonal Antibody (CD19/3116).

img

Formalin-fixed, paraffin-embedded human Tonsil stained with CD19 Monospecific Mouse Monoclonal Antibody (CD19/3116).

img

Flow Cytometric Analysis of Raji cells using CD19 Monospecific Mouse Monoclonal Antibody (CD19/3116) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

img

SDS-PAGE Analysis Purified CD19 Monospecific Mouse Monoclonal Antibody (CD19/3116). Confirmation of Integrity and Purity of Antibody.

img

Analysis of Protein Array containing more than 19,000 full-length human proteins using CD19 Monospecific Mouse Monoclonal Antibody (CD19/3116).

Product Details

Synonyms

B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; CVID3; Leu-12; T-cell surface antigen Leu-12

Positive Control

Raji cells (FACS). Tonsil, Lymph Node or Spleen (IHC).

Known Applications & Suggested Dilutions

ELISA, Flow Cyt, IHC-P         More Details

ELISA (For coating, order antibody without BSA)
Flow Cytometry (1-2ug/million cells)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of human CD19 protein (around aa96-281) (exact sequence is proprietary)

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

95kDa

Specificity & Comments

CD19 is a transmembrane glycoprotein that contains two extracellular immunoglobulin-like domains. CD19 is present in both benign and malignant B-cells and is considered to be the most reliable surface marker of this lineage over a wide range of maturational stages. In normal lymphoid tissue, CD19 is observed in germinal centers, in mantle zone cells, and in scattered cells of the inter-follicular areas. Anti-CD19 exhibits an overall immunoreactivity pattern similar to those of the antibodies against CD20 and CD22. However, in contrast to CD20, expression of CD19 is continuous throughout B-cell development and through terminal differentiation of B-cells into plasma cells. Anti-CD19 positivity is seen in the vast majority of B-cell neoplasms commonly at a lower intensity than normal B-cell counterparts. Plasma cell neoplasms are nearly always negative, as are T-cell neoplasms.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Bregni, M., et al.1989. Blood 73: 753-762

Bioinformatics

  •  Entrez Gene ID: 930
  •  Gene Symbol: CD19
  •  SwissProt: P15391
  •  Chromosome Location: 16p11.2
  •  Unigene: 652262
NBT03116