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Home >> Antibodies >> CD147 / EMMPRIN / Neurothelin

CD147 / EMMPRIN / Neurothelin

Mouse Monoclonal Antibody [Clone 8D6]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
img

Formalin-fixed, paraffin-embedded human renal cell carcinoma stained with CD147 Mouse Monoclonal Antibody (8D6).

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SDS-PAGE Analysis Purified CD147 Mouse Monoclonal Antibody (8D6). Confirmation of Integrity and Purity of Antibody.

Product Details

Synonyms

5A11 Antigen; 5F7; Basigin; Blood brain barrier HT7 antigen BSG; Extracellular Matrix Metalloproteinase Inducer (EMMPRIN); Leukocyte Activation Antigen M6; Neurothelin; OK Blood Group Antigen; TCSF; Tumor Cell-derived Collagenase Stimulatory Factor

Positive Control

HSB2 cells. Human renal cell carcinoma, ovarian carcinoma or melanoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Inhibits Homotypic Aggregation, Adhesion to Matrix Proteins, & Migation through Matrigel (Order w/o BSA / azide)
Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Human T cell line Molt 13

Cellular Localization

Cell surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

35kDa (non-reduced); 40kDa (reduced)

Specificity & Comments

This MAb recognizes extracellular epitope 2 within the N-terminal Ig domain of human CD147. It is expressed more intensely on thymocytes than on mature peripheral blood T cells. CD147 is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. It stimulates the production of interstitial collagenase, gelatinase A, stromelysin-1 and various metalloproteinases (MMPs) by fibroblasts. These enzymes are important factors in cancer invasion and metastasis.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Kirsch AH et. al. Tissue Antigens. 1997 Aug;50(2):147-52.

Bioinformatics

  •  Entrez Gene ID: 682
  •  Gene Symbol: BSG
  •  SwissProt: P35613
  •  Chromosome Location: 19p13.3
  •  Unigene: 501293