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Recombinant CD147 / EMMPRIN / Neurothelin Antibody [BSG/9223R]

In Stock
Catalog Number Formulation Size Price
682-RBM20-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
682-RBM20-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
682-RBM20-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
SDS-PAGE Analysis CD147 Recombinant Rabbit Monoclonal Antibody (BSG/9223R). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis CD147 Recombinant Rabbit Monoclonal Antibody (BSG/9223R). Confirmation of Purity and Integrity of Antibody.

Formalin-fixed, paraffin-embedded human placenta stained with CD147 Recombinant Rabbit Monoclonal Antibody (BSG/9223R). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human placenta stained with CD147 Recombinant Rabbit Monoclonal Antibody (BSG/9223R). Inset: PBS instead of primary antibody; secondary only negative control.

CD147 Antibody in Western Blot (WB)

CD147 Antibody in Western Blot (WB)

Western Blot Analysis of HepG2 cell lysate using CD147 Recombinant Rabbit Monoclonal Antibody (BSG/9223R).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

This MAb recognizes extracellular epitope 2 within the N-terminal Ig domain of human CD147. It is expressed more intensely on thymocytes than on mature peripheral blood T cells. CD147 is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. It stimulates the production of interstitial collagenase, gelatinase A, stromelysin-1 and various metalloproteinases (MMPs) by fibroblasts. These enzymes are important factors in cancer invasion and metastasis.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular, Infectious Disease
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell surface.
Gene Name
BSG
Positive Control
HepG2, HeLa. Human renal cell carcinoma
Immunogen
Recombinant full-length human BSG protein
Alternate Names
Basigin, 5F7, Collagenase stimulatory factor, Extracellular matrix metalloproteinase inducer, Hepatoma-associated antigen, Leukocyte activation antigen M6, OK blood group antigen, Tumor cell-derived collagenase stimulatory factor, 5A11 Antigen; 5F7; Basigin; Blood brain barrier HT7 antigen BSG; Extracellular Matrix Metalloproteinase Inducer (EMMPRIN); Leukocyte Activation Antigen M6; Neurothelin; OK Blood Group Antigen; TCSF; Tumor Cell-derived Collagenase Stimulatory Factor

Database Links

Entrez Gene ID
682
SwissProt
P35613

Additional Information

Clone
BSG/9223R
Chromosome Location
19p13.3
Mol. Weight of Antigen
35kDa (non-reduced); 40kDa (reduced)

Related Antibodies

View All BSG Antibodies

Functions

  • Essential for normal retinal maturation and development (By similarity). Acts as a retinal cell surface receptor for NXNL1 and plays an important role in NXNL1-mediated survival of retinal cone photoreceptors (PubMed:25957687). In association with glucose transporter SLC16A1/GLUT1 and NXNL1, promotes retinal cone survival by enhancing aerobic glycolysis and accelerating the entry of glucose into photoreceptors (PubMed:25957687). May act as a potent stimulator of IL6 secretion in multiple cell lines that include monocytes (PubMed:21620857).
  • (Microbial infection) Erythrocyte receptor for P.falciparum RH5 which is essential for erythrocyte invasion by the merozoite stage of P.falciparum isolates 3D7 and Dd2.
  • Signaling receptor for cyclophilins, essential for PPIA/CYPA and PPIB/CYPB-dependent signaling related to chemotaxis and adhesion of immune cells (PubMed:11943775, PubMed:11688976). Plays an important role in targeting monocarboxylate transporters SLC16A1/GLUT1, SLC16A11 and SLC16A12 to the plasma membrane (PubMed:17127621, PubMed:21778275, PubMed:28666119). Acts as a coreceptor for vascular endothelial growth factor receptor 2 (KDR/VEGFR2) in endothelial cells enhancing its VEGFA-mediated activation and downstream signaling (PubMed:25825981). Promotes angiogenesis through EPAS1/HIF2A-mediated up-regulation of VEGFA (isoform VEGF-165 and VEGF-121) and KDR/VEGFR2 in endothelial cells (PubMed:19837976). Plays a key role in regulating tumor growth, invasion, metastasis and neoangiogenesis by stimulating the production and release of extracellular matrix metalloproteinases and KDR/VEGFR2 by both tumor cells and stromal cells (fibroblasts and endothelial cells) (PubMed:12553375, PubMed:11992541, PubMed:15833850).
  • (Microbial infection) Erythrocyte receptor for P.falciparum RH5 which is essential for erythrocyte invasion by the merozoite stage of P.falciparum isolates 3D7, Dd2, 7G8 and HB3 (PubMed:22080952, PubMed:26195724). Binding of P.falciparum RH5 results in BSG dimerization which triggers an increase in intracellular Ca(2+) in the erythrocyte (PubMed:28409866). This essential step leads to a rearrangement of the erythrocyte cytoskeleton required for the merozoite invasion (PubMed:28409866).
  • (Microbial infection) Can facilitate human SARS coronavirus (SARS-CoV-1) infection via its interaction with virus-associated PPIA/CYPA.
  • (Microbial infection) Can facilitate HIV-1 infection via its interaction with virus-associated PPIA/CYPA.
  • (Microbial infection) First described as a receptor for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), it is not required for SARS-CoV-2 infection.
  • (Microbial infection) Acts as a receptor for measles virus.
  • (Microbial infection) Promotes entry of pentamer-expressing human cytomegalovirus (HCMV) into epithelial and endothelial cells.

Key References

  • Kirsch AH et. al. Tissue Antigens. 1997 Aug;50(2):147-52.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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