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Home >> Antibodies >> CD11b / MAC-1 (Microglial Marker)

CD11b / MAC-1 (Microglial Marker)

Mouse Monoclonal Antibody [Clone ITGAM/3340]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human tonsil stained with CD11b Monospecific Mouse Monoclonal Antibody (ITGAM/3340).

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Formalin-fixed, paraffin-embedded human tonsil stained with CD11b Monospecific Mouse Monoclonal Antibody (ITGAM/3340).

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SDS-PAGE Analysis Purified CD11b Monospecific Mouse Monoclonal Antibody (ITGAM/3340). Confirmation of Purity and Integrity of Antibody.

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Western Blot Analysis of human spleen tissue lysate using CD11b Monospecific Mouse Monoclonal Antibody (ITGAM/3340).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using CD11b Monospecific Mouse Monoclonal Antibody (ITGAM/3340).

Product Details

Synonyms

CD18; CD49d; Cell surface glycoprotein MAC-1 subunit alpha; Complement Component Receptor 3 Alpha; CR3 Alpha Chain (CR3A); Integrin alpha-M; Integrin beta 2 alpha subunit; Leukocyte adhesion receptor MO1; Ly-40; MAC1A; Macrophage antigen alpha polypeptide; MO1A; Neutrophil adherence receptor alpha M subunit

Positive Control

Monocytes & granulocytes. Lymph nodes, spleen and tonsil (IHC).

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa941-1074) of human ITGAM protein (exact sequence is proprietary)

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

95kDa & 170kDa

Specificity & Comments

CD11b is a cell adhesion molecule that acts as a receptor for cell surface ligands such as intracellular adhesion molecules (ICAMs) or soluble ligands. Integrins are heterodimeric proteins that contain an a chain and b chain. Integrin aM combines with the Integrin '2 to form a leukocyte-specific integrin referred to as macrophage receptor 1 (Mac-1), or inactivated-C3b (iC3b) receptor 3 (CR3). Integrin aM/'2 is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. The protein CD11b has been implicated in the various adhesion-related interactions of cells such as monocytes, macrophages, natural killer (NK) cells, and granulocytes. It is part of a heterodimer that consists of CD11b andCD18. It also modulates the uptake of complement-coated particles within the cell. It is commonly used as a microglial marker in tissues derived from the nervous system.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Springer T, et al. 1978. Eur. J. Immunol. 8:539.

Bioinformatics

  •  Entrez Gene ID: 3684
  •  Gene Symbol: ITGAM
  •  SwissProt: P11215
  •  Chromosome Location: 16p11.2
  •  Unigene: 172631
NBT03340