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Home >> Antibodies >> Calprotectin / MRP14 / S100A9 / Calgranulin B

Calprotectin / MRP14 / S100A9 / Calgranulin B

Mouse Monoclonal Antibody [Clone 47-8D3; same as DF3973]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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SDS-PAGE Analysis Purified Calprotectin Monoclonal Antibody (DF3973). Confirmation of Integrity and Purity of Antibody.

Product Details

Synonyms

S100-A9; MRP14; calgranulin-B; calprotectin L1H subunit; leukocyte L1 complex heavy chain; migration inhibitory factor-related protein 14; S100 calcium-binding protein A9 (calgranulin B)

Positive Control

Liver or histiocytoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(For staining of formalin-fixed, paraffin-embedded tissues, digest with trypsin at 1mg/ml PBS, 15 min at RT Optimal dilution for a specific application should be determined.

Immunogen

Human peripheral blood monocyte components

Cellular Localization

Cytoplasmic, Extracellular (secreted) and Cell Surface

Species Reactivity

Human. Rat.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

14kDa

Specificity & Comments

This MAb stains the cytoplasm of macrophages and histiocytes in hematopoietic organs, Kupffer's cells of the liver and Langerhan's cells of the skin. It also stains the mantle zone B-lymphocytes of the lymph node and spleen, spermatogonia, and chief cells of the stomach. S100A9 is expressed by macrophages in acutely inflamed tissues and in chronic inflammation. It is detected in peripheral blood leukocytes, in neutrophils and granulocytes. It is present at sites of vascular inflammation. S100A9 is also expressed in epithelial cells constitutively or induced during dermatoses. S100A9 is a Calcium-binding protein. It has antimicrobial activity towards bacteria and fungi. It is important for resistance to invasion by pathogenic bacteria. It up-regulates transcription of genes that are under the control of NF-kappa-B. S100A9 plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS). It promotes tubulin polymerization when unphosphorylated. It also promotes phagocyte migration and infiltration of granulocytes at sites of wounding. It plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% azide. Also available WITHOUT azide at 1.0mg/ml.

Key References

  1. Flavell DJ. et al., J. Histochem. Cytochem. 35, 12171226, 1987.
  2. Bardadin KA. et al., J. Pathol. 164, 253259, 1991.
  3. Facchetti F. et al., Am. J. Clin. Pathol. 92, 4250, 1989.
  4. Goebeler M. Et al., J. Leukocyte Biol. 55, 259-261, 1994.

Bioinformatics

  •  Entrez Gene ID: 6280
  •  Gene Symbol: S100A9
  •  SwissProt: P06702
  •  Unigene: 112405
NBT01093