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Home >> Antibodies >> Calponin-1 (Smooth Muscle Marker)

Calponin-1 (Smooth Muscle Marker)

Mouse Monoclonal Antibody [Clone CNN1/832]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Calponin-1 Mouse Monoclonal Antibody (CNN1/832).

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Formalin-fixed, paraffin-embedded human Uterus stained with Calponin-1 Mouse Monoclonal Antibody (CNN1/832).

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Formalin-fixed, paraffin-embedded Rat Uterus stained with Calponin-1 Mouse Monoclonal Antibody (CNN1/832).

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SDS-PAGE Analysis Purified Calponin-1 Mouse Monoclonal Antibody (CNN1/832). Confirmation of Purity and Integrity of Antibody

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Immunofluorescence Analysis of PFA-fixed K562 cells labeling Calponin using Calponin-1 Mouse Monoclonal Antibody (CNN1/832).followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Redot.

Product Details

Synonyms

Calponin 1 basic smooth muscle; Calponin H1 smooth muscle; Calponin-1; CNN1; Cnn1; Sm Calp; SMCC

Positive Control

K562 cells, Myoepithelial cells in breast ducts.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human CNN1 protein

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Rat.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

34kDa

Specificity & Comments

Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist; a 34kDa form and a 29kDa form. Expression of the 29kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this MAb reacts with only the 34kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Tang, D.C., et al. 1996. Structure-function relations of smooth muscle Calponin. The critical role of Serine 175. J. Biol. Chem. 271: 8605-8611.

Bioinformatics

  •  Entrez Gene ID: 1264
  •  Gene Symbol: CNN1
  •  SwissProt: P51911
  •  Chromosome Location: 19p13.2
  •  Unigene: 465929
NBT00832