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Home >> Antibodies >> Actin, Smooth Muscle (Leiomyosarcoma Marker)

Actin, Smooth Muscle (Leiomyosarcoma Marker)

Mouse Monoclonal Antibody [Clone SPM332]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/m

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/m

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with Smooth Muscle Actin Mouse Monoclonal Antibody (SPM332).

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SDS-PAGE Analysis Purified Smooth Muscle Actin Mouse Monoclonal Antibody (SPM322). Confirmation of Integrity and Purity of Antibody.

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Immunofluorescence Analysis of HeLa cells labeling Smooth Muscle Actin with Smooth Muscle Actin Mouse Monoclonal Antibody (SPM322) followed by Goat anti-Mouse IgG-CF488(Green). The nuclear counterstain is NucSpot (Red).

Product Details

Synonyms

ACTA2, Actin Alpha 2 Smooth Muscle Aorta, Actin Aortic Smooth Muscle, Actin Vascular Smooth Muscle, ACTSA, ACTVS, Alpha 2 Actin, Alpha Actin 2, Alpha Cardiac Actin, Alpha Smooth Muscle, Alpha-actin-2, Aortic Smooth Muscle, ASMA, Cell Growth-inhibiting Gene 46 Protein, Growth Inhibiting Gene 46

Positive Control

Blood vessels in all tissues, smooth muscle or leiomyosarcoma.

Known Applications & Suggested Dilutions

IF, IHC-P         More Details

Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

N-Terminal decapeptide of alpha smooth muscle isoform of actin and conjugated to KLH.

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Baboon. Monkey. Cow. Pig. Sheep. Goat. Cat. Dog. Rabbit. Mouse. Rat. Guinea Pig. Chicken.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

42kDa

Specificity & Comments

Actin is a major component of the cytoskeleton and is present in most cell types. This MAb is highly specific to actin from smooth muscles. Its epitope lies in the first four N-terminal amino acids. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Skalli O et. al. Journal of Cell Biology, 1986, 103:2787-96.

Bioinformatics

  •  Entrez Gene ID: 59
  •  Gene Symbol: ACTA2
  •  SwissProt: P62736
  •  Chromosome Location: 10q23.31
  •  Unigene: 500483
NBT00665