Home >> Antibodies >> Actin, Muscle Specific (Muscle Cell Marker)

Actin, Muscle Specific (Muscle Cell Marker)

Recombinant Mouse Monoclonal Antibody [Clone rMSA/953]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

SDS-PAGE Analysis Purified MSA Recombinant Mouse Monoclonal Antibody (rMSA/953). Confirmation of Purity and Integrity of Antibody.

Western blot analysis of human skeletal muscle tissue lysate using Muscle Specific Actin Recombinant Mouse Monoclonal Antibody (rMSA/953).

SDS-PAGE Analysis Purified MSA Recombinant Mouse Monoclonal Antibody (rMSA/953). Confirmation of Purity and Integrity of Antibody.

Western blot analysis of human skeletal muscle tissue lysate using Muscle Specific Actin Recombinant Mouse Monoclonal Antibody (rMSA/953).

Product Details

Synonyms

ACTA, ACTA1, ACTA2, ACTC1, Actin, ACTSA, Alpha-2 actin, alpha skeletal muscle, Alpha-actin-1, Cardiac muscle alpha actin-1, Skeletal muscle alpha actin-1

Positive Control

Muscle or sarcoma.

Known Applications & Suggested Dilutions

Western Blot, IHC-P More Details

Western Blot (0.5-1.0ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant protein corresponding to human muscle actin

Cellular Localization

Cytoplasm.

Species Reactivity

Human, Rabbit and Rat.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

42kDa

Specificity & Comments

This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).