Recombinant von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) [VWF/7979R]

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Summary

Human von Willebrand factor (VWF or factor VIII-related antigen) is a 309 kDa multimeric plasma glycoprotein, important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Von Willebrand factor also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma. Von Willebrand factor is synthesized by endothelial cells and is reported to be expressed in a number of tumors of vascular origin. IHC results aid in the classification of acute myeloid leukemia FAB type M7, angiosarcoma and epithelioid hemangioendothelioma.

Product Properties & Targets

Host
Species Reactivity
Isotype
Cellular Localization
Cytoplasm.
Gene Name
VWF
Positive Control
Human tonsil.
Immunogen
Recombinant fragment (around aa 1150-1250) of human vWF protein (exact sequence is proprietary)
Alternate Names
von Willebrand factor | von Willebrand antigen 2, von Willebrand antigen II, Coagulation Factor VIII, F8VWF, von Willebrand Antigen 2, von Willebrand Disease (vWD)

Database Links

Entrez Gene ID
Unigene
SwissProt

Additional Information

Clone
VWF/7979R
Human Gene Symbol
VWF
Chromosome Location
12p13.31
Mol. Weight of Antigen
250kDa

Functions

  • Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.

Known Applications & Suggested Dilutions

  • Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

  • Motta, A.et al.2009. J Biomater Sci Polym Ed. 20: 1875-1897.
  • Germann, B.et al.2008. Pharmazie. 63: 303-307.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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