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Recombinant TRIM29 (Lung Squamous Cell Carcinoma Marker) Antibody [TRIM29/9257R]

In Stock
Catalog Number Formulation Size Price
23650-RBM7-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
23650-RBM7-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
23650-RBM7-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

It recognizes a 66kDa protein, which is identified as Tripartite motif-containing protein 29 (TRIM29). It interacts with the intermediate filament protein vimentin, a substrate for the PKC family of protein kinases, and with hPKCI-1, an inhibitor of the PKCs. TRIM29 protein contains both zinc finger and leucine zipper motifs, suggesting that the it may form homodimers and possibly associate with DNA. High expression of TRIM29 has been reported in gastric cancer and pancreatic cancer, and correlates with enhanced tumor growth and lymph node metastasis. TRIM29 is also able to distinguish lung squamous cell carcinoma from lung adenocarcinoma with ~90% positive accuracy, when used in a panel with TTF-1, p63, CK5/6, and Napsin-A antibodies.

Product Properties & Targets

Host
Rabbit
Applications
Species Reactivity
Research Areas
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cytoplasmic. Cell surface.
Gene Name
Positive Control
A431 cells. Human tonsil or squamous cell carcinoma.
Immunogen
Recombinant fragment (around aa 1-200) of human TRIM29 protein (exact sequence is proprietary)
Alternate Names
Tripartite motif-containing protein 29, Ataxia telangiectasia group D-associated protein, Ataxia telangiectasia group D complementing gene (ATDC); Tripartite motif-containing protein 29 (TRIM29);

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
TRIM29/9257R
Chromosome Location
11q23.3
Mol. Weight of Antigen
66kDa

Functions

  • Plays a crucial role in the regulation of macrophage activation in response to viral or bacterial infections within the respiratory tract. Mechanistically, TRIM29 interacts with IKBKG/NEMO in the lysosome where it induces its 'Lys-48' ubiquitination and subsequent degradation. In turn, the expression of type I interferons and the production of pro-inflammatory cytokines are inhibited. Additionally, induces the 'Lys-48' ubiquitination of STING1 in a similar way, leading to its degradation.

Key References

  • Kosaka Y, et al. Tripartite motif-containing 29 (TRIM29) is a novel marker for lymph node metastasis in gastric cancer. Ann Surg Oncol. 14(9): 2543-9. 2007.
  • Ring BZ, et al. A novel five-antibody immunohistochemical test for sub-classification of lung carcinoma. Mod Pathol. 22(8): 1032-43. 2009.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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