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TRIM29 (Lung Squamous Cell Carcinoma Marker) Antibody [MSVA-629M]

In Stock
Catalog Number Formulation Size Price
23650-MSM8-P0
Purified Ab with BSA and Azide
20ug
$324.50
23650-MSM8-P1
Purified Ab with BSA and Azide
100ug
$599.50
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
TRIM29 Antibody in Numerous Tissues (IHC)

TRIM29 Antibody in Numerous Tissues (IHC)

Tripartite motif-containing protein 29 Mouse Monoclonal Antibody (MSVA-629M) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1:100-1:200
IHC Protocol
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Summary

It recognizes a 66kDa protein, which is identified as Tripartite motif-containing protein 29 (TRIM29). It interacts with the intermediate filament protein vimentin, a substrate for the PKC family of protein kinases, and with hPKCI-1, an inhibitor of the PKCs. TRIM29 protein contains both zinc finger and leucine zipper motifs, suggesting that the it may form homodimers and possibly associate with DNA. High expression of TRIM29 has been reported in gastric cancer and pancreatic cancer, and correlates with enhanced tumor growth and lymph node metastasis. TRIM29 is also able to distinguish lung squamous cell carcinoma from lung adenocarcinoma with ~90% positive accuracy, when used in a panel with TTF-1, p63, CK5/6, and Napsin-A antibodies.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Immunology, Cytokine Signaling
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Cytoplasm, Lysosome
Gene Name
TRIM29
Positive Control
Tonsil: A strong membranous and cytoplasmic TRIM29 immunostaining should be seen in squamous epithelial cells.
Immunogen
Recombinant fragment (126 Amino acid residues between aa 1-200) of human TRIM29 protein
Alternate Names
Tripartite motif-containing protein 29, Ataxia telangiectasia group D-associated protein, Ataxia telangiectasia group D complementing gene (ATDC); Tripartite motif-containing protein 29 (TRIM29);

Database Links

Entrez Gene ID
23650
SwissProt
Q14134

Additional Information

Clone
MSVA-629M
Chromosome Location
11q23.3
Mol. Weight of Antigen
66kDa

Related Antibodies

View All TRIM29 Antibodies

Functions

  • Plays a crucial role in the regulation of macrophage activation in response to viral or bacterial infections within the respiratory tract. Mechanistically, TRIM29 interacts with IKBKG/NEMO in the lysosome where it induces its 'Lys-48' ubiquitination and subsequent degradation. In turn, the expression of type I interferons and the production of pro-inflammatory cytokines are inhibited. Additionally, induces the 'Lys-48' ubiquitination of STING1 in a similar way, leading to its degradation.

Key References

  • Kosaka Y, et al. Tripartite motif-containing 29 (TRIM29) is a novel marker for lymph node metastasis in gastric cancer. Ann Surg Oncol. 14(9): 2543-9. 2007.
  • Ring BZ, et al. A novel five-antibody immunohistochemical test for sub-classification of lung carcinoma. Mod Pathol. 22(8): 1032-43. 2009.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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