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Recombinant Synaptophysin (Neuroendocrine Marker) Antibody [rSYP/6856]

In Stock
Catalog Number Formulation Size Price
6855-MSM6-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
6855-MSM6-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
6855-MSM6-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human brain stained with rSYP/6856 at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min. Inset: PBS instead of primary. Secondary antibody negative control.

Formalin-fixed, paraffin-embedded human brain stained with rSYP/6856 at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min. Inset: PBS instead of primary. Secondary antibody negative control.

SDS-PAGE Analysis of Purified Synaptophysin Recombinant Mouse Monoclonal Antibody (rSYP/6856). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis of Purified Synaptophysin Recombinant Mouse Monoclonal Antibody (rSYP/6856). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

This recombinant mouse monoclonal antibody recognizes a protein of 38kDa that is identified as synaptophysin. It is an N-glycosylated integral membrane protein found in neurons and endocrine cells. Synaptophysin contains four transmembrane domains and may function as a gap junction-like channel. This antibody identifies normal neuroendocrine cells and neuroendocrine neoplasms. Diffuse, finely granular, cytoplasmic staining is observed, which probably correlates with the distribution of the antigen within neurosecretory vesicles. Synaptophysin is an independent, broad-range marker of neural and neuroendocrine differentiation.

Product Properties & Targets

Antibody Type
Mouse Monoclonals, Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Neuroscience, Neural Stem Cells
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell junction, Cytoplasmic vesicle, Secretory vesicle, Synapse, Synaptic vesicle membrane, Synaptosome
Gene Name
SYP
Positive Control
Cerebellum or Pheochromocytoma., HeLa or Y79 cells. Human pancreas, HePG2
Immunogen
Recombinant fragment (around aa 224-313) of human Synaptophysin (SYP) protein (exact sequence is proprietary)
Alternate Names
Synaptophysin, Major synaptic vesicle protein p38, Major synaptic vesicle protein p38; MRX96; MRXSYP; Syn p38; Syp; SYPH

Database Links

Entrez Gene ID
6855
SwissProt
IDP08247

Additional Information

Clone
rSYP/6856
Chromosome Location
Xp11.23
Mol. Weight of Antigen
38kDa

Related Antibodies

View All SYP Antibodies

Functions

  • Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity).

Key References

  • Wiedenmann B, et al. Identification and localization of synaptophysin, an integral membrane glycoprotein of Mr 38,000 characteristic of presynaptic vesicles. Cell. 1985; 41:1017-28.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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