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Recombinant Synaptophysin (Neuroendocrine Marker) Antibody [MSVA-462R]

In Stock
Catalog Number Formulation Size Price
6855-RBM25-P0
Purified Ab with BSA and Azide
20ug
$324.50
6855-RBM25-P1
Purified Ab with BSA and Azide
100ug
$599.50
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
SYP Antibody in Numerous Tissues (IHC)

SYP Antibody in Numerous Tissues (IHC)

Synaptophysin Rabbit Recombinant Monoclonal Antibody (MSVA-462R) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1:100-1:200
IHC Protocol
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Summary

This recombinant rabbit monoclonal antibody recognizes a protein of 38kDa that is identified as synaptophysin. It is an N-glycosylated integral membrane protein found in neurons and endocrine cells. Synaptophysin contains four transmembrane domains and may function as a gap junction-like channel. This antibody identifies normal neuroendocrine cells and neuroendocrine neoplasms. Diffuse, finely granular, cytoplasmic staining is observed, which probably correlates with the distribution of the antigen within neurosecretory vesicles. Synaptophysin is an independent, broad-range marker of neural and neuroendocrine differentiation.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Neuroscience, Neural Stem Cells
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell junction, Cytoplasmic vesicle, Secretory vesicle, Synapse, Synaptic vesicle membrane, Synaptosome
Gene Name
SYP
Positive Control
Colon: A moderate to strong staining of the axons of the Auerbach’s and Meissner’s plexus should be seen while the endocrine cells of the mucosa and the peripheral nerves in the mucosa must show an at least a weak to moderate staining. Pancreas: A strong staining should be seen in islets of Langerhans and in peripheral nerve fibres located between the exocrine cells.
Immunogen
Recombinant fragment (around aa 274-313) of human Synaptophysin (SYP) protein (exact sequence is proprietary)
Alternate Names
Synaptophysin, Major synaptic vesicle protein p38, Major synaptic vesicle protein p38; MRX96; MRXSYP; Syn p38; Syp; SYPH

Database Links

Entrez Gene ID
6855
SwissProt
P08247

Additional Information

Clone
MSVA-462R
Chromosome Location
Xp11.23
Mol. Weight of Antigen
38kDa

Related Antibodies

View All SYP Antibodies

Functions

  • Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity).

Key References

  • Wiedenmann B, et al. Identification and localization of synaptophysin, an integral membrane glycoprotein of Mr 38,000 characteristic of presynaptic vesicles. Cell. 1985; 41:1017-28.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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