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Recombinant PU.1 (SPI-1) (B-Cell Marker) Antibody [rPU1/2146]

In Stock
Catalog Number Formulation Size Price
6688-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
6688-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
6688-MSM3-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant PU.1 (SPI-1) (B-Cell Marker) Antibody

Formalin-fixed, paraffin-embedded human lymph node stained with PU.1 Recombinant Mouse Monoclonal Antibody (rPU1/2146).

Recombinant PU.1 (SPI-1) (B-Cell Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human lymph node stained with PU.1 Recombinant Mouse Monoclonal Antibody (rPU1/2146).

Recombinant PU.1 (SPI-1) (B-Cell Marker) Antibody - Image 3

SDS-PAGE Analysis of Purified PU.1 Recombinant Mouse Monoclonal Antibody (rPU1/2146). Confirmation of Purity and Integrity of Antibody.

PU.1 Antibody in Western Blot (WB)

PU.1 Antibody in Western Blot (WB)

Western Blot Analysis of Raji cell lysate using PU.1-Monospecific Recombinant Mouse Monoclonal Antibody (rPU1/2146).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

PU.1 is a member of the ETS family of transcription factors and is important for normal B-cell development. It is expressed in the myeloid lineage and in immature as well as mature B-lymphocytes, with the exception of plasma cells. PU.1 is expressed in germinal center B-cells and mantle B-cells. Various lymphomas are also positive for this marker. It is essential during early B-cell differentiation. The absence of PU.1 results in total block of B-cell development at the pre-pro stage. PU.1 plays a key role in normal myeloid differentiation, and regulates the expression of immunoglobulin and other genes that are important for B-cell development.

Product Properties & Targets

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Nucleus
Gene Name
SPI1
Positive Control
Colon carcinoma., Hodgkin s Lymphoma, K562 or THP-1 cells. Lymph Node, Ramos, Spleen, Raji.
Immunogen
Recombinant fragment (around aa16-170) of the human PU.1 protein (exact sequence is proprietary)
Alternate Names
Transcription factor PU.1, AGM10|OF|PU.1|SFPI1|SPI-1|SPI-A

Database Links

Entrez Gene ID
6688
SwissProt
P17947

Additional Information

Clone
rPU1/2146
Chromosome Location
11p11.2
Mol. Weight of Antigen
31.08kDa
Expression System
HEK293 Cells
Purification
Protein G

Related Antibodies

View All SPI1 Antibodies

Functions

  • Pioneer transcription factor, which controls hematopoietic cell fate by decompacting stem cell heterochromatin and allowing other transcription factors to enter otherwise inaccessible genomic sites. Once in open chromatin, can directly control gene expression by binding genetic regulatory elements and can also more broadly influence transcription by recruiting transcription factors, such as interferon regulatory factors (IRFs), to otherwise inaccessible genomic regions (PubMed:23658224, PubMed:33951726). Transcriptionally activates genes important for myeloid and lymphoid lineages, such as CSF1R (By similarity). Transcriptional activation from certain promoters, possibly containing low affinity binding sites, is achieved cooperatively with other transcription factors. FCER1A transactivation is achieved in cooperation with GATA1 (By similarity). May be particularly important for the pro- to pre-B cell transition (PubMed:33951726). Binds (via the ETS domain) onto the purine-rich DNA core sequence 5'-GAGGAA-3', also known as the PU-box (PubMed:33951726). In vitro can bind RNA and interfere with pre-mRNA splicing (By similarity).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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