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Recombinant IgG4 (Fc) (Ig Heavy Constant Gamma 4) Antibody [IGHG4/13367R]

In Stock
Catalog Number Formulation Size Price
3503-RBM5-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
3503-RBM5-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
3503-RBM5-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
IgG4 Antibody in Immunohistochemistry (IHC (P))

IgG4 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human tonsil stained with IgG4 (Fc) Recombinant Rabbit Monoclonal Antibody (IGHG4/13367R). Inset: PBS instead of primary antibody; secondary only negative control.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

IgG4-related sclerosing disease has been recognized as a systemic disease entity characterized by an elevated serum IgG4 level, sclerosing fibrosis, and diffuse lymphoplasmacytic infiltration with the presence of many IgG4-positive plasma cells. Clinical manifestations are apparent in the pancreas, bile duct, gall bladder, lacrimal gland, salivary gland, retroperitoneum, kidney, lung, breast, thyroid, and prostate. Immunohistochemical analyses in the case of IgG4-related sclerosing disease not only exhibit significantly more than normal IgG4-positive plasma cells in affected tissues but also significantly higher IgG4/IgG ratios (typically > 30%).
IgG4 antibodies will dominate the IgG response in schistosomiasis, lymphatic filariasis, and in patients after allergen immunotherapy. Unlike the other IgG subclasses, IgG4 does not activate complement. A combined IgA-IgG4 deficiency has been associated with recurrent pyogenic infections.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
B Cell Markers
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell membrane, Secreted
Gene Name
IGHG4
Positive Control
Human tonsil.
Immunogen
Recombinant fragment (around aa1-200) of human IGHG4 protein (exact sequence is proprietary)
Alternate Names
Immunoglobulin heavy constant gamma 4, Ig gamma-4 chain C region, Ig gamma 4 chain C region; IGHG4; Immunoglobulin heavy constant gamma 4 (G4m marker)

Database Links

Entrez Gene ID
3503
SwissProt
P01861

Additional Information

Clone
IGHG4/13367R
Chromosome Location
14q32.33
Mol. Weight of Antigen
36kDa

Related Antibodies

View All IGHG4 Antibodies

Functions

  • Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:22158414, PubMed:20176268). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268).

Key References

  • Adetugbo, K., et al. 1978. Evolution of immunoglobulin subclasses. Primary structure of a murine myeloma 1 chain. J. Biol. Chem. 253: 6068-6075.
  • Li J et al. (2020) Mol Med Rep 22:371-379 (2020).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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