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Recombinant Collagen IV Antibody [MSVA-704R]

In Stock
Catalog Number Formulation Size Price
1282-RBM8-P0
Purified Ab with BSA and Azide
20ug
$324.50
1282-RBM8-P1
Purified Ab with BSA and Azide
100ug
$599.50
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
COL4A1 Antibody in Numerous Tissues (IHC)

COL4A1 Antibody in Numerous Tissues (IHC)

Collagen alpha-1(IV) chain Rabbit Recombinant Monoclonal Antibody (MSVA-704R) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1:100-1:200
IHC Protocol
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Summary

Collagen Type IV is a major component of the basement membrane and plays an important role in cell adhesion, migration, differentiation and growth. Collagen Type IV express at the basement membranes in a variety of tissues including kidney, muscle, lymph nodes, lung, tendon and spleen. Collagen Type IV has been shown to be useful in differentiating microinvasive from in situ ductal carcinomas of the breast. Other Collagen Type IV studies include use in pancreatic adenocarcinoma and chronic pancreatitis, nephrosclerosis and other kidney diseases, oral squamous cell carcinoma, laryngeal cancers, ovarian cancers and cervical cancers.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Lung Cancer, Signal Transduction
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Basement membrane, Extracellular matrix, Extracellular space, Secreted
Gene Name
COL4A1
Positive Control
Colon: An at least moderate collagen IV immunostaining of basement membranes and a strong staining of vessels and the tunica muscularis mucosa should be seen.
Immunogen
Purified human placental extract
Alternate Names
Collagen alpha-1(IV) chain, Arresten; BSVD; COL4A1; COL4A1 NC1 domain; COL4A2; COL4A3; COL4A4; COL4A5; collagen alpha-1(IV) chain; Collagen IV Alpha 1 Polypeptide; Collagen IV Alpha 2 Polypeptide; Collagen Type IV Alpha 2; Collagen Type IV Alpha 3; Collagen Type IV Alpha 4; Collagen Type IV Alpha 5; RATOR

Database Links

Entrez Gene ID
1282 (COL4A1); 1284 (COL4A2); 1285 (COL4A3); 1286 (COL4A4); 1287 (COL4A5)
SwissProt
P02462& P08572; P29400; P53420

Additional Information

Clone
MSVA-704R
Chromosome Location
13q34 (COL4A1 & COL4A2); 2q36.3 (COL4A3 & COL4A4); Xq22.3 (COL4A5)
Mol. Weight of Antigen
161kDa
Expression System
HEK293 Cells

Related Antibodies

View All COL4A1 Antibodies

Functions

  • Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
  • Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation.

Key References

  • Smrkolj S et al. Eur J Gynaecol Oncol. 2010; 31(4):380- 5.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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