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p53 Tumor Suppressor Protein Antibody [DO-7]

In Stock
Catalog Number Formulation Size Price
7157-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
7157-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
7157-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
p53 Tumor Suppressor Protein Antibody

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with p53 Mouse Monoclonal Antibody (DO-7).

p53 Tumor Suppressor Protein Antibody - Image 2

Western Blot Analysis of human HeLa cell lysate using p53 Mouse Monoclonal Antibody (DO-7).

p53 Tumor Suppressor Protein Antibody - Image 3

SDS-PAGE Analysis of Purified p53 Mouse Monoclonal Antibody (DO-7). Confirmation of Integrity and Purity of Antibody

TP53 Antibody in SDS-PAGE

TP53 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Cellular tumor antigen p53 Recombinant Mouse Monoclonal Antibody (DO-7). Confirmation of Purity and Integrity of Antibody.

p53 Antibody in Western Blot (WB)

p53 Antibody in Western Blot (WB)

Western Blot Analysis of Raji cell lysate using p53 Mouse Monoclonal Antibody (DO-7).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Recognizes a 53kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53. Its epitope maps within the N-terminus (aa 37-45) of p53. Monoclonal antibody PAb1801 does not block the binding of DO-7 MAb to p53 in an ELISA test. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Cow, Human, Monkey
Research Areas
Apoptosis, Breast Cancer, Cancer, Cardiovascular, Hypoxia, Immunology, AKT Signaling, Bladder Cancer, Colon Cancer, Cytokine Signaling, Defective Intrinsic Apoptosis, Infectious Disease, Lung Cancer, MAPK Signaling, Nuclear Marker, Ovarian Cancer, Signal Transduction, Transcription Factors
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Centrosome, Cytoplasm, Cytoskeleton, Endoplasmic reticulum, Microtubule organizing center, Mitochondrion matrix, Nucleus, PML body
Gene Name
TP53
Positive Control
MDA-MB-231 cells. Breast or Colon carcinoma. Raji.
Immunogen
Recombinant human wild type p53 protein expressed in E. coli.
Alternate Names
Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, Antigen NY-CO-13, BCC7, Cellular Tumor Antigen p53, LFS1, TP53, Transformation Related Protein 53 (TRP53), Tumor Protein p53, Tumor Suppressor p53

Database Links

Entrez Gene ID
7157
SwissProt
P04637

Additional Information

Clone
DO-7
Chromosome Location
17p13.1
Mol. Weight of Antigen
53kDa.
Expression System
CHO Cells
Purification
Protein A

Related Antibodies

View All TP53 Antibodies

Functions

  • Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type (PubMed:11025664, PubMed:12524540, PubMed:12810724, PubMed:15186775, PubMed:15340061, PubMed:17317671, PubMed:17349958, PubMed:19556538, PubMed:20673990, PubMed:20959462, PubMed:22726440, PubMed:24051492, PubMed:9840937, PubMed:24652652). Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process (PubMed:11025664, PubMed:12524540, PubMed:12810724, PubMed:15186775, PubMed:15340061, PubMed:17317671, PubMed:17349958, PubMed:19556538, PubMed:20673990, PubMed:20959462, PubMed:22726440, PubMed:24051492, PubMed:9840937, PubMed:24652652). One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 (PubMed:12524540). However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP (PubMed:12524540). In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seems to have an effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492).

Key References

  • Stephen CW et al. 1995. J Mol. Biol. 248(1): 58-78.
  • Vojtesek B et al. 1992. J. Immunol. Methods. 151(1-2): 237-44.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use diagnosis.

Supplied as

200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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