Formalin-fixed, paraffin-embedded human Ewing's Sarcoma stained with NKX2.2 Rabbit Recombinant Monoclonal Antibody (NX2/1422R).

SDS-PAGE Analysis Purified NKX2.2 Rabbit Recombinant Monoclonal Antibody (NX2/1422R).

Printable Datasheet

NKX2.2 (Neuroendocrine & Ewing’s Sarcoma Marker) [NX2/1422R]

Name: NKX2.2 (Neuroendocrine &amp; Ewing's Sarcoma Marker)
SKU: 4821-RBM2
Availability: InStock

Monoclonals Primary Antibodies Rabbit Monoclonals Recombinant Rabbit Monoclonals Recombinants

Summary

Expression of NKX2.2 has been found in neuroendocrine tumors of the gut, making it a potential marker for the study of gastrointestinal neuroendocrine tumors. More recently, NKX2.2 protein was identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. It acts as a valuable marker for Ewing sarcoma, with a sensitivity of 93% and a specificity of 89%, and aids in the differential diagnosis of small round cell tumors.

Product Properties & Targets

Host

Rabbit

Species Reactivity

Human

Research Areas

Autophagy, Cardiovascular

Isotype

IgG

Cellular Localization

Nucleus

Gene Name

NKX2-2

Mol. Weight of Antigen

40-50kDa

Immunogen

Recombinant human full-length NKX2.2 protein

Database Links

Entrez Gene ID

4821

Unigene

516922

SwissProt

O95096

Additional Information

Clone

NX2/1422R

Human Gene Symbol

NKX2-2

Chromosome Location

20p11.22

Known Applications & Suggested Dilutions

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Key References

Wang, Y.C. et al. (2009) Endocr Relat Cancer 16, 267-79.
Yoshida A et. al. Am J Surg Pathol. 2012;36(7):993-9.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.