Formalin-fixed, paraffin-embedded human Melanoma stained with NGFR Rabbit Recombinant Monoclonal Antibody (NGFR/2550R).

SDS-PAGE Analysis Purified NGFR Rabbit Recombinant Monoclonal Antibody (NGFR/2550R). Confirmation of Integrity and Purity of Antibody.

Western Blot Analysis of U87 cell lysate usingNGFR Rabbit Recombinant Monoclonal Antibody (NGFR/2550R).

Printable Datasheet

NGF-Receptor (p75) / CD271 (Soft Tissue Tumor Marker) [NGFR/2550R]

Name: NGF-Receptor (p75) / CD271 (Soft Tissue Tumor Marker)
SKU: 4804-RBM9
Availability: InStock

Monoclonals Primary Antibodies Rabbit Monoclonals Recombinant Rabbit Monoclonals Recombinants

Summary

It recognizes a glycoprotein of 75kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). NGFR is expressed in various neural crest cells and their tumors such as melanocytes, melanomas, neuroblastomas, pheochromocytomas and neurofibromas. Reportedly, anti-NGFR is a reliable marker for desmoplastic and neurotropic melanomas. NGFR is expressed in mature non-neural cells such as perivascular cells, dental pulp cells, lymphoidal follicular dendritic cells, basal epithelium of oral mucosa and hair follicles, prostate basal cells, and myoepithelial cells. Anti-NGFR stains the myoepithelial cells of breast ducts and intra-lobular fibroblasts of breast ducts.

Product Properties & Targets

Host

Rabbit

Species Reactivity

Human

Isotype

IgG

Cellular Localization

Cell membrane, Cell projection, Dendritic spine, Growth cone, Perikaryon

Gene Name

NGFR

Mol. Weight of Antigen

75kDa

Immunogen

Recombinant human p75 NGFR protein fragment (around aa 281-421) (exact sequence is proprietary)

Database Links

Entrez Gene ID

4804

Unigene

415768; 681726

SwissProt

P08138

Additional Information

Clone

NGFR/2550R

Human Gene Symbol

NGFR

Chromosome Location

17q21-22

Known Applications & Suggested Dilutions

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Key References

Soland, T.M., et al. 2008. Nerve growth factor receptor (p75NTR) and pattern of invasion predict poor prognosis in oral squamous cell carcinoma. Histopathology 53: 62-72.
Wang, W., et al. 2009. Patterns of expression and function of the p75NGFR protein in pancreatic cancer cells and tumours. Eur. J. Surg. Oncol. 35: 826-832.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.