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Recombinant MUC3 (Mucin 3) Antibody [rMUC3/1154]

In Stock
Catalog Number Formulation Size Price
4584-MSM4-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
4584-MSM4-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
4584-MSM4-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human gastric carcinoma stained with MUC3 Recombinant Mouse Monoclonal Antibody (rMUC3/1154). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human gastric carcinoma stained with MUC3 Recombinant Mouse Monoclonal Antibody (rMUC3/1154). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human gastric carcinoma stained with MUC3 Recombinant Mouse Monoclonal Antibody (rMUC3/1154). HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human gastric carcinoma stained with MUC3 Recombinant Mouse Monoclonal Antibody (rMUC3/1154). HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

It recognizes a protein of HMW, identified as mucin 3 glycoprotein (MUC3). Its epitope localizes between aa SITTTE. This MAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC2. MUC3 is distributed in colon and rectum, and is also present to a lesser extent in breast, lung and salivary gland tissues.The Mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs) encoded by 60 base pairs (Mucin 1), 69 base pairs (Mucin 2) and 51 base pairs (Mucin 3). The number of repeats is highly polymorphic and varies among different alleles. Mucin 1 proteins are expressed as type I membrane proteins in addition to secreted forms. Mucin 1 is aberrantly expressed in epithelial tumors including breast carcinomas. Mucin 2 coats the epithelia of the intestines and airways and is associated with colonic tumors. Mucin 3 is a major component of various mucus gels and is broadly expressed in normal and tumor cells.

Product Properties & Targets

Antibody Type
Mouse Monoclonals, Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Immunology, Infectious Disease
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Membrane, Secreted
Gene Name
MUC3A
Positive Control
LS174T cells.Human colon or colon carcinoma.
Immunogen
Recombinant fragment of human MUC3A protein (exact sequence is proprietary)
Alternate Names
Mucin-3A, Intestinal mucin-3A, Intestinal mucin-3A; intestinal mucin-like; MUC3; MUC3A; MUC3B; mucin 3, intestinal; mucin 3A, cell surface associated; mucin 3A, intestinal; mucin 3B, cell surface associated

Database Links

Entrez Gene ID
4584& 57876
SwissProt
Q02505

Additional Information

Clone
rMUC3/1154
Chromosome Location
7q22.1
Mol. Weight of Antigen
1100kDa

Related Antibodies

View All MUC3A Antibodies

Functions

  • Major glycoprotein component of a variety of mucus gels. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. May be involved in ligand binding and intracellular signaling.

Key References

  • Apostolopoulos V, et. al. Journal of Gastroenterology and Hepatology, 1995, 10:555-61.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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