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MUC3 (Mucin 3) Antibody [MUC3/1154]

Additional information

Catalog Number: 4584-MSM2
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
4584-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
4584-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
4584-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
MUC3 (Mucin 3) Antibody

Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC3 Monoclonal Antibody (MUC3/1154).

MUC3 (Mucin 3) Antibody - Image 2

Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC3 Monoclonal Antibody (MUC3/1154).

MUC3 (Mucin 3) Antibody - Image 3

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MUC3 Monoclonal Antibody (MUC3/1154).

MUC3A Antibody in SDS-PAGE

MUC3A Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Mucin-3A Mouse Monoclonal Antibody (MUC3/1154). Confirmation of Purity and Integrity of Antibody.

  • Flow
  • IF
  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Human
Clone
MUC3/1154
Isotype
IgG2a
Light Chain
Kappa
Cellular Localization
Membrane, Secreted
Gene Name
MUC3A
Positive Control
LS174T cells. Human small intestine (IHC).
Immunogen
A synthetic peptide of 35 amino acids, SIB35 (C-HSTPSFTSSITTTETTSHSTPSFTSSITTTETTS), which contains two of the MUC3 tandem repeats, coupled to KLH.
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Major glycoprotein component of a variety of mucus gels. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. May be involved in ligand binding and intracellular signaling.
Key References
  • Gum, J.R. Jr., et al. 1990. Molecular cloning of cDNAs derived from a novel human intestinal Mucin gene. Biochem. Biophys. Res. Commun 171: 407-415.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
4584; 57876
SwissProt
Q02505; Q9H195
Chromosome Location
7q22.1
Mol. Weight of Antigen
1100kDa
Alternate Names
Mucin-3A, Intestinal mucin-3A, Intestinal mucin-3A; intestinal mucin-like; MUC3; MUC3A; MUC3B; mucin 3, intestinal; mucin 3A, cell surface associated; mucin 3A, intestinal; mucin 3B, cell surface associated

Summary

It recognizes a protein of HMW, identified as mucin 3 glycoprotein (MUC3). This MAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC2. The Mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs) encoded by 60 base pairs (Mucin 1), 69 base pairs (Mucin 2) and 51 base pairs (Mucin 3). The number of repeats is highly polymorphic and varies among different alleles. Mucin 1 proteins are expressed as type I membrane proteins in addition to secreted forms. Mucin 1 is aberrantly expressed in epithelial tumors including breast carcinomas. Mucin 2 coats the epithelia of the intestines and airways and is associated with colonic tumors. Mucin 3 is a major component of various mucus gels and is broadly expressed in normal and tumor cells.

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