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Recombinant MSH2 (DNA Mismatch Repair Marker) Antibody [MSH2/6549R]

In Stock
Catalog Number Formulation Size Price
4436-RBM11-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
4436-RBM11-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
4436-RBM11-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human colonstained with MSH2Recombinant Rabbit Monoclonal Antibody (MSH2/6549R).

Formalin-fixed, paraffin-embedded human colonstained with MSH2Recombinant Rabbit Monoclonal Antibody (MSH2/6549R).

Formalin-fixed, paraffin-embedded human colon stained with MSH2Recombinant Rabbit Monoclonal Antibody (MSH2/6549R).

Formalin-fixed, paraffin-embedded human colon stained with MSH2Recombinant Rabbit Monoclonal Antibody (MSH2/6549R).

Western blot analysis of HCT116 cell lysate using MSH2Recombinant Rabbit Monoclonal Antibody (MSH2/6549R).

Western blot analysis of HCT116 cell lysate using MSH2Recombinant Rabbit Monoclonal Antibody (MSH2/6549R).

SDS-PAGE Analysis of Purified MSH2Recombinant Rabbit Monoclonal Antibody (MSH2/6549R). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis of Purified MSH2Recombinant Rabbit Monoclonal Antibody (MSH2/6549R). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC). Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes MutS and MutL were found at high frequency in HNPCC and were shown to be associated with microsatellite instability. The demonstration that 10 to 45% of pancreatic, gastric, breast, ovarian and small cell lung cancers also display microsatellite instability has been interpreted to suggest that DNA mismatch repair is not restricted to HNPCC tumors but is a common feature in tumor initiation or progression.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Colon Cancer, Infectious Disease, Nuclear Marker, Transcription Factors
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Chromosome, Nucleus
Gene Name
MSH2
Positive Control
A549 or HepG2 cells. Human colon, Testis or Lymph Node., Thyroid
Immunogen
Recombinant full-length human MSH2 protein
Alternate Names
DNA mismatch repair protein Msh2, MutS protein homolog 2, BAT26; COCA1; DNA mismatch repair protein Msh2; FCC1; hMSH2; HNPCC1; LCFS2; MSH2; MutS homolog 2; MutS homolog 2 colon cancer nonpolyposis type 1; MutS protein homolog 2

Database Links

Entrez Gene ID
4436
SwissProt
IDP43246

Additional Information

Clone
MSH2/6549R
Chromosome Location
2p21-p16.3
Mol. Weight of Antigen
100kDa

Related Antibodies

View All MSH2 Antibodies

Functions

  • Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. Recruits DNA helicase MCM9 to chromatin which unwinds the mismatch containing DNA strand (PubMed:26300262). ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP
  • >ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.

Key References

  • Palombo, F., et al. 1994. Mismatch repair and cancer. Nature 367: 417-418.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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