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MSH2 (DNA Mismatch Repair Marker) Antibody [MSH2/2622]

Additional information

Catalog Number: 4436-MSM4
Reactivity:

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Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
4436-MSM4-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
4436-MSM4-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
4436-MSM4-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
MSH2 (DNA Mismatch Repair Marker) Antibody

Formalin-fixed, paraffin-embedded human Colon from Lynch disease stained with MSH2 Mouse Monoclonal Antibody (MSH2/2622).

MSH2 (DNA Mismatch Repair Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human Thyroid Carcinoma stained with MSH2 Mouse Monoclonal Antibody (MSH2/2622).

MSH2 (DNA Mismatch Repair Marker) Antibody - Image 3

Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with MSH2 Mouse Monoclonal Antibody (MSH2/2622).

MSH2 (DNA Mismatch Repair Marker) Antibody - Image 4

Western Blot Analysis of human HepG2, A549, and A431 cell lysate using MSH2 Mouse Monoclonal Antibody (MSH2/2622).

MSH2 (DNA Mismatch Repair Marker) Antibody - Image 5

Flow Cytometric Analysis of A549 cells using MSH2 Mouse Monoclonal Antibody (MSH2/2622) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

MSH2 (DNA Mismatch Repair Marker) Antibody - Image 6

SDS-PAGE Analysis of Purified MSH2 Mouse Monoclonal Antibody (MSH2/2622). Confirmation of Purity and Integrity of Antibody

MSH2 (DNA Mismatch Repair Marker) Antibody - Image 7

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MSH2 Mouse Monoclonal Antibody (MSH2/2622).

MSH2 (DNA Mismatch Repair Marker) Antibody - Image 8

Immunofluorescence staining of MOLT-4 cells using MSH2 Mouse Monoclonal Antibody (MSH2/2622) followed by goat anti-mouse IgG conjugated to CF488 (green). Membrane are stained with Phalloiden (Red).

MSH2 Antibody in Western Blot (WB)

MSH2 Antibody in Western Blot (WB)

Western blot analysis of Human Testis, Mouse Testis, Rat Testis, Hamster Testis, Guinea pig Testis, K-562 and HeLa lysates using MSH2 Mouse Monoclonal Antibody (MSH2/2622).

  • Flow
  • IF
  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Dog, Guinea Pig, Hamster, Human, Mouse, Rat
Clone
MSH2/2622
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Chromosome, Nucleus
Gene Name
MSH2
Positive Control
A549 or HepG2 cells. Colon, Testis or Lymph Node., Thyroid, Human Testis, Mouse Testis, Rat Testis, Hamster Testis, Guinea pig Testis, K-562 or HeLa
Immunogen
Recombinant fragment (around aa 327-427) of human MSH2 protein (exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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TOM1L1 (Target of Myb1 Like 1 Membrane Trafficking Protein) Antibody

Additional information

Catalog Number: 10040-MSM1
Reactivity:

Gene:

Host:

Formalin-fixed, paraffin-embedded human Kidney stained with Occludin Mouse Monoclonal Antibody (OCLN/2183).
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Occludin (OCLN) (Tight Junctions Marker) Antibody

Additional information

Catalog Number: 100506658-MSM3
Reactivity:

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Host:

Formalin-fixed, paraffin-embedded Mouse Heart stained with N-Cadherin Mouse Monoclonal Antibody (CDH2/1573).
1
  • IHC

N-Cadherin / Cadherin-2 / CD325 (NCAD) Antibody

Additional information

Catalog Number: 1000-MSM3
Reactivity:

,

Gene:

Host:

Flow cytometric analysis of PFA-fixed HeLa cells. MEF2B Mouse Monoclonal Antibody (PCRP-MEF2B-2F9) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).
2
  • Flow
  • IF
  • WB

MEF2B (Transcriptional Activator) Antibody

Additional information

Catalog Number: 100271849-MSM1
Reactivity:

Gene:

Host:

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with KSP-Cadherin Mouse Monoclonal Antibody (SPM594).
2
  • IHC
  • WB

Ksp-Cadherin (Kidney-Specific Cadherin) / CDH16 Antibody

Additional information

Catalog Number: 1014-MSM1X
Reactivity:

, , , ,

Gene:

Host:

Formalin-fixed, paraffin-embedded human Colon stained with Cadherin 17 / CDH17 Mouse Monoclonal Antibody (CDH17/2618).
4
  • Flow
  • IHC
  • WB

Cadherin 17 / LI Cadherin (Gastric Adenocarcinoma Marker) Antibody

Additional information

Catalog Number: 1015-MSM8
Reactivity:

Gene:

Host:

Formalin-fixed, paraffin-embedded human Colon stained with Cadherin 17 / CDH17 Mouse Monoclonal Antibody (CDH17/2616).
3
  • IHC
  • WB

Cadherin 17 / LI Cadherin (Gastric Adenocarcinoma Marker) Antibody

Additional information

Catalog Number: 1015-MSM6
Reactivity:

Gene:

Host:

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with KSP-Cadherin Rabbit Recombinant Monoclonal Antibody (CDH16/1532R)
6
  • IHC
  • WB
  • Flow

Recombinant Ksp-Cadherin (Kidney-Specific Cadherin) / CDH16 Antibody

Additional information

Catalog Number: 1014-RBM2
Gene:

Host:

Reactivity:

VIEW ALL ANTIBODIES

Other Information

Functions
  • Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. Recruits DNA helicase MCM9 to chromatin which unwinds the mismatch containing DNA strand (PubMed:26300262). ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP
  • >ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.
Key References
  • Palombo, F., et al. 1994. Mismatch repair and cancer. Nature 367: 417-41
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
4436
SwissProt
IDP43246
Chromosome Location
2p21-p16.3
Mol. Weight of Antigen
100kDa
Alternate Names
DNA mismatch repair protein Msh2, MutS protein homolog 2, BAT26; COCA1; DNA mismatch repair protein Msh2; FCC1; hMSH2; HNPCC1; LCFS2; MSH2; MutS homolog 2; MutS homolog 2 colon cancer nonpolyposis type 1; MutS protein homolog 2

Summary

Mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC). Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes MutS and MutL were found at high frequency in HNPCC and were shown to be associated with microsatellite instability. The demonstration that 10 to 45% of pancreatic, gastric, breast, ovarian and small cell lung cancers also display microsatellite instability has been interpreted to suggest that DNA mismatch repair is not restricted to HNPCC tumors but is a common feature in tumor initiation or progression.

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