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Mitochondrial Marker Antibody [SPM198]

In Stock
Catalog Number Formulation Size Price
MSM1X-739-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
MSM1X-739-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
MSM1X-739-P1ABX
Purified Ab WITHOUT BSA at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Mitochondrial Marker Antibody

Western Blot Analysis of HeLa cell lysate using Mitochondria Mouse Monoclonal Antibody (SPM198).

Mitochondrial Marker Antibody - Image 2

Confocal immunofluorescence image of HeLa cells using Mitochondria Mouse Monoclonal Antibody (SPM198) labeled in Green (CF488) and Reddot is used to label the nuclei purple.

Mitochondrial Marker Antibody - Image 3

Formalin-fixed, paraffin-embedded human Pancreas stained with Mitochondria Mouse Monoclonal Antibody (SPM198).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

MAb 113-1 recognizes a 60kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
N/A
Gene Name
N/A
Positive Control
HeLa or HepG2 cells. Hepatic carcinoma.
Immunogen
Semi-Purified mitochondrial preparation
Alternate Names
N/A, N/A, Not Known

Database Links

Entrez Gene ID
Not Known
SwissProt
N/A

Additional Information

Clone
SPM198
Chromosome Location
Not Known
Mol. Weight of Antigen
60kDa

Related Antibodies

View All N/A Antibodies

Functions

  • N/A

Key References

  • Epstein, Alan L. and Clevenger, Charles V. In: Progress in Nonhistone Protein Research, Vol. 1, Isaac Bekhor, Ed. CRC Press, Boca Raton, FL, pp. 117-137, 1985.

PubMed Links

  • N/A

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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