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Recombinant Mammaglobin (SCGB2A2) (Breast Cancer Marker) Antibody [MGB/2682R]

In Stock
Catalog Number Formulation Size Price
4250-RBM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
4250-RBM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
4250-RBM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Mammaglobin (SCGB2A2) (Breast Cancer Marker) Antibody

Formalin-fixed, paraffin-embedded human breast carcinoma stained with Mammaglobin Recombinant Rabbit Monoclonal Antibody (MGB/2682R).

Recombinant Mammaglobin (SCGB2A2) (Breast Cancer Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human breast carcinoma stained with Mammaglobin Recombinant Rabbit Monoclonal Antibody (MGB/2682R).

Recombinant Mammaglobin (SCGB2A2) (Breast Cancer Marker) Antibody - Image 3

Immunofluorescence Analysis of MCF-7 cells using Mammaglobin Recombinant Rabbit Monoclonal Antibody (MGB/2682R) followed by goat anti-mouse IgG-CF488; counterstained with RedDot.

Mammaglobin Antibody in Western Blot (WB)

Mammaglobin Antibody in Western Blot (WB)

Western Blot Analysis of SK-BR-3, T-47D and Daudi cell lysates using MammaglobinRecombinant Rabbit Monoclonal Antibody (MGB/2682R).

Mammaglobin Antibody in Western Blot (WB)

Mammaglobin Antibody in Western Blot (WB)

Western Blot Analysis of Daudi cell lysate using Mammaglobin Recombinant Rabbit Monoclonal Antibody (MGB/2682R).

SCGB2A2 Antibody in SDS-PAGE

SCGB2A2 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Mammaglobin-A Recombinant Rabbit Monoclonal Antibody (MGB/2682R). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Mammaglobin is a 93 amino acid glycoprotein with homology to other secretoglobin-uteroglobin family members. It was originally identified as a breast cancer restricted biomarker by differential screening. Mammaglobin related to secretoglobin family that includes human uteroglobin and lipophilin. Mammaglobin antibody stains cytoplasm of normal breast epithelial cells as well as primary and metastatic breast carcinomas. Mammaglobin expression is absent in prostate, kidney, colon, rectum, small intestine, stomach, pancreas, lung, and thyroid tissues.Mammaglobin may be used as part of an immunohistochemical panel for determination of metastatic breast carcinoma and tumor of unknown primary origin.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Secreted
Gene Name
SCGB2A2
Positive Control
HeLa or MCF-7, SK-BR-3, T-47D, Daudi cells. Mammary gland specific. Overexpressed in breast cancer.
Immunogen
Recombinant full-length human Mammaglobin (SCGB2A2) protein
Alternate Names
Mammaglobin-A, Mammaglobin-1, Secretoglobin family 2A member 2, Mammaglobin-1 (MGB1); Mammaglobin-A; Secretoglobin family 2A member 2 (SCGB2A2); SG2A2; UGB2

Database Links

Entrez Gene ID
4250
SwissProt
Q13296

Additional Information

Clone
MGB/2682R
Chromosome Location
11q12.3
Mol. Weight of Antigen
18-25kDa

Related Antibodies

View All SCGB2A2 Antibodies

Key References

  • Watson MA., et al. Cancer Res. 1999;59:3028-3031.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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