Western Blot Analysis of human HeLa cell lysate using Major Vault Protein (MVP) Mouse Monoclonal Antibody (Clone 1014).
Immunofluorescence Analysis of A549 cells labeling MVP with MVP Monoclonal Antibody (Clone 1014) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).
SDS-PAGE Analysis Purified MVP Monoclonal Antibody (Clone 1014). Confirmation of Purity and Integrity of Antibody.
Recognizes a protein of 104kDa-110kDa, characterized as major vault protein (MVP). Vaults are large ribonucleoprotein particles (RNPs) present in all eukaryotic cells. They have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for 70% of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy.
