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Recombinant CD163 (Monocyte & Macrophage Marker) Antibody [VP2897R]

Additional information

Catalog Number: 9961-RBM3
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
9961-RBM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
9961-RBM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
9961-RBM3-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant CD163 (Monocyte & Macrophage Marker) Antibody

Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Major Vault Protein Rabbit Recombinant Monoclonal Antibody (VP2897R).

Major Vault Protein (MVP) Antibody in Western Blot (WB)

Major Vault Protein (MVP) Antibody in Western Blot (WB)

Western Blot Analysis of A549 lysate using Major Vault Protein (MVP) Rabbit Recombinant Monoclonal Antibody (VP2897R).

Major Vault Protein Antibody in Western Blot (WB)

Major Vault Protein Antibody in Western Blot (WB)

Western Blot Analysis of Human Heart, Human Liver, Human Lung and Human Spleen tissue lysates using Major Vault Protein Recombinant Rabbit Monoclonal Antibody (VP2897R).

Major Vault Protein Antibody in Western Blot (WB)

Major Vault Protein Antibody in Western Blot (WB)

Western Blot Analysis of Kidney, Spleen and Stomach tissue lysates of different species using Major Vault Protein Recombinant Rabbit Monoclonal Antibody (VP2897R).

MVP Antibody in SDS-PAGE

MVP Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Major vault protein Rabbit Monoclonal Antibody (VP2897R). Confirmation of Purity and Integrity of Antibody.

MVP Antibody in SDS-PAGE

MVP Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Major vault protein Rabbit Monoclonal Antibody (VP2897R). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
VP2897R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cytoplasm, Nuclear pore complex, Nucleus, Perinuclear region
Gene Name
MVP
Positive Control
A549, MCF-7 or HeLa cells. Breast tumors, Human Heart, Human Liver, Human Lung, Human Spleen
Immunogen
Recombinant full-length human Major Vault Protein (MVP) protein
Expression System
HEK293
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates IFNG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
9961
SwissProt
Q14764
Chromosome Location
16p11.2
Mol. Weight of Antigen
99.33kDa
Alternate Names
Major vault protein, LRP|VAULT1

Summary

Recognizes a protein of 104kDa-110kDa, characterized as major vault protein (MVP). Vaults are large ribonucleoprotein particles (RNPs) present in all eukaryotic cells. They have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for 70% of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy.

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