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Recombinant LMO2 (B-Cell Marker) Antibody [LMO2/3147R]

In Stock
Catalog Number Formulation Size Price
4005-RBM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
4005-RBM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
4005-RBM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant LMO2 (B-Cell Marker) Antibody

Immunofluorescence staining of K562 cells using LMO2 Recombinant Rabbit Monoclonal Antibody (LMO2/3147R) followed by goat anti-rabbit IgG-CF488 (green). Membrane stained with Phalloidin (Red).

Recombinant LMO2 (B-Cell Marker) Antibody - Image 2

Flow Cytometric Analysis of K562 cells using LMO2 Recombinant Rabbit Monoclonal Antibody (LMO2/3147R); followed by goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).

Recombinant LMO2 (B-Cell Marker) Antibody - Image 3

SDS-PAGE Analysis Purified LMO2 Recombinant Rabbit Monoclonal Antibody (LMO2/3147R). Confirmation of Purity and Integrity of Antibody.

LMO2 Antibody in Western Blot (WB)

LMO2 Antibody in Western Blot (WB)

Western Blot Analysis of Human Brain tissue lysate using LMO2 Recombinant Rabbit Monoclonal Antibody (LMO2/3147R).

LMO2 Antibody in Western Blot (WB)

LMO2 Antibody in Western Blot (WB)

Western Blot Analysis of Human Brain, Human Skeletal Muscle, K-562, Raji and Ramos lysates using LMO2 Recombinant Rabbit Monoclonal Antibody (LMO2/3147R).

LMO2 Antibody in Western Blot (WB)

LMO2 Antibody in Western Blot (WB)

Western Blot Analysis of Human Brain, Mouse Brain, Rat Brain, Hamster Brain, Guinea pig Brain, Rabbit Brain, Human Skeletal Muscle, Mouse Skeletal Muscle, Rat Skeletal Muscle, Hamster Skeletal Muscle, Guinea Pig Skeletal Muscle and Rabbit Skeletal Muscle tissue lysates using LMO2 Recombinant Rabbit Monoclonal Antibody (LMO2/3147R).

Applications & Dilutions

Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

The LMO2 protein has a central and crucial role in hematopoietic development and is highly conserved. It has a particular function in normal and lymphatic endothelial cells involving the regulation of angiogenesis and lymph-angiogenesis. Immunohistochemical studies have also demonstrated expression of LMO2 in both normal germinal center B-cells and germinal center-derived B-cell lymphomas, including follicular lymphoma and diffuse large B-cell lymphoma.The use of anti-LMO2 is valuable as a tool in the identification of lymphomas of B-cell origin. LMO2 is useful in differentiating follicular lymphoma (LMO2+) from nodal marginal zone lymphoma (LM02-). It also is positive in Hodgkin s and Burkitt s lymphomas.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Flow Cytometry, Immunofluorescence, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular, Nuclear Marker, Stem Cell Differentiation, Transcription Factors
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Nucleus
Gene Name
LMO2
Positive Control
K562, Hodgkin s lymphoma, Ramos or Raji cells. Human placenta, pancreas, Brain, Human Skeletal Muscle
Immunogen
Recombinant fragment (around aa 23-140) human LMO2 protein (exact sequence is proprietary)
Alternate Names
Rhombotin-2, Cysteine-rich protein TTG-2, LIM domain only protein 2, T-cell translocation protein 2, Cysteine-rich protein TTG-2; LIM domain only protein 2; LMO-2; RBTN L1; RBTN2; Rhombotin like 1; Rhombotin-2 (RHOM2); T-cell translocation protein 2; TTG2

Database Links

Entrez Gene ID
4005
SwissProt
P25791

Additional Information

Clone
LMO2/3147R
Chromosome Location
11p13
Mol. Weight of Antigen
24kDa

Related Antibodies

View All LMO2 Antibodies

Functions

  • Acts with TAL1/SCL to regulate red blood cell development. Also acts with LDB1 to maintain erythroid precursors in an immature state.

Key References

  • Younes, SF et al. Am J Clin Pathol. 2011 May;135(5):697-708.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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