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Recombinant LEF1 / TCF1 alpha (Transcription Factor) Antibody [LEF1/9403R]

In Stock
Catalog Number Formulation Size Price
51176-RBM10-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
51176-RBM10-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
51176-RBM10-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant LEF1 / TCF1 alpha (Transcription Factor) Antibody

Formalin-fixed, paraffin-embedded analysis of human tonsil. Strong nuclear staining of non-germinal center cells using LEF1/9403R. Inset: PBS instead of primary antibody; secondary only negative control.

Recombinant LEF1 / TCF1 alpha (Transcription Factor) Antibody - Image 2

SDS-PAGE Analysis of Purified LEF1 Recombinant Rabbit Monoclonal Antibody (LEF1/9403R. Confirmation of Purity and Integrity of Antibody.

LEF1 Antibody in Western Blot (WB)

LEF1 Antibody in Western Blot (WB)

Western Blot analysis of Jurkat cell lysate using LEF1 Recombinant Rabbit Monoclonal Antibody (LEF1/9403R).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Lymphoid Enhancing Factor 1 (LEF1) is a transcription factor that belongs to the TCF/LEF family. LEF1 participates as a regulator in Wnt signaling pathways. LEF1 is an important factor in lymphopoiesis and is expressed normally in T and pro-B cells but not expressed in mature B cells. Anti-LEF1 may be used as an aid for differentiation of chronic lymphocytic leukemia/small lymphocytic lymphoma from other small B cell lymphomas.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Breast Cancer, Cardiovascular, Developmental Biology, BBB VCAM-1 Signaling, Colon Cancer, Endothelial Cell Marker, Nuclear Marker, Signal Transduction, Transcription Factors
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Nucleus.
Gene Name
LEF1
Positive Control
Human tonsil and thymus.
Immunogen
Recombinant fragment (around aa1-200) of human LEF1 protein (exact sequence is proprietary)
Alternate Names
Lymphoid enhancer-binding factor 1, T cell-specific transcription factor 1-alpha, Lymphoid enhancer-binding factor 1 (LEF1); T cell specific transcription factor 1 alpha (TCF1a); TCF1 alpha; TCF10; TCF7L3; Transcription factor T cell specific 1 alpha

Database Links

Entrez Gene ID
51176
SwissProt
Q9UJU2

Additional Information

Clone
LEF1/9403R
Chromosome Location
4q25
Mol. Weight of Antigen
44kDa

Related Antibodies

View All LEF1 Antibodies

Functions

  • Transcription factor that binds DNA in a sequence-specific manner (PubMed:2010090). Participates in the Wnt signaling pathway (By similarity). Activates transcription of target genes in the presence of CTNNB1 and EP300 (By similarity). PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1 (PubMed:11266540). Regulates T-cell receptor alpha enhancer function (PubMed:19653274). Required for IL17A expressing gamma-delta T-cell maturation and development, via binding to regulator loci of BLK to modulate expression (By similarity). May play a role in hair cell differentiation and follicle morphogenesis (By similarity).
  • Transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.
  • Lacks the CTNNB1 interaction domain and may therefore be an antagonist for Wnt signaling.
  • Transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells.

Key References

  • Ji Q, et al. Nat Commun 10:313 (2019).
  • Przybyl J, et al. Gynecol Oncol 149:388-393 (2018).

PubMed Links

Storage & Stability

Antibody with azide store at 2 to 8°C. Antibody without azide store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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