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Recombinant LEF1 / TCF1 alpha (Transcription Factor) Antibody [rLEF1/6906]

In Stock
Catalog Number Formulation Size Price
51176-MSM6-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
51176-MSM6-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
51176-MSM6-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Lymphoid Enhancing Factor 1 (LEF1) is a transcription factor that belongs to the TCF/LEF family. LEF1 participates as a regulator in Wnt signaling pathways. LEF1 is an important factor in lymphopoiesis and is expressed normally in T and pro-B cells but not expressed in mature B cells. Anti-LEF1 may be used as an aid for differentiation of chronic lymphocytic leukemia/small lymphocytic lymphoma from other small B cell lymphomas.

Product Properties & Targets

Host
Mouse
Applications
Species Reactivity
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Nucleus
Gene Name
Positive Control
Human tonsil and thymus.
Immunogen
Recombinant fragment (around aa100-200) of human LEF1 protein (exact sequence is proprietary)
Alternate Names
Lymphoid enhancer-binding factor 1, T cell-specific transcription factor 1-alpha, Lymphoid enhancer-binding factor 1 (LEF1); T cell specific transcription factor 1 alpha (TCF1a); TCF1 alpha; TCF10; TCF7L3; Transcription factor T cell specific 1 alpha

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
rLEF1/6906
Chromosome Location
4q25
Mol. Weight of Antigen
44kDa

Functions

  • Transcription factor that binds DNA in a sequence-specific manner (PubMed:2010090). Participates in the Wnt signaling pathway (By similarity). Activates transcription of target genes in the presence of CTNNB1 and EP300 (By similarity). PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1 (PubMed:11266540). Regulates T-cell receptor alpha enhancer function (PubMed:19653274). Required for IL17A expressing gamma-delta T-cell maturation and development, via binding to regulator loci of BLK to modulate expression (By similarity). May play a role in hair cell differentiation and follicle morphogenesis (By similarity).
  • Transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.
  • Lacks the CTNNB1 interaction domain and may therefore be an antagonist for Wnt signaling.
  • Transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells.

Key References

  • Ji Q, et al. Nat Commun 10:313 (2019).
  • Przybyl J, et al. Gynecol Oncol 149:388-393 (2018).

Storage & Stability

Antibody with azide store at 2 to 8 °C. Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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