IHC analysis of formalin-fixed, paraffin-embedded human tonsil. Strong nuclear staining of non-germinal center cells using LEF1/341R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.Inset: PBS instead of primary. Secondary antibody negative control.

IHC analysis of formalin-fixed, paraffin-embedded human lymph node. Strong nuclear staining of non-germinal center cells using LEF1/341R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

Printable Datasheet

LEF1 / TCF1 alpha (Transcription Factor) [LEF1/341R]

Name: LEF1 / TCF1 alpha (Transcription Factor)
SKU: 51176-RBM1
Availability: InStock

Monoclonals Primary Antibodies Rabbit Monoclonals Recombinant Rabbit Monoclonals Recombinants

Summary

Lymphoid Enhancing Factor 1 (LEF1) is a transcription factor that belongs to the TCF/LEF family. LEF1 participates as a regulator in Wnt signaling pathways. LEF1 is an important factor in lymphopoiesis and is expressed normally in T and pro-B cells but not expressed in mature B cells. Anti-LEF1 may be used as an aid for differentiation of chronic lymphocytic leukemia/small lymphocytic lymphoma from other small B cell lymphomas.

Product Properties & Targets

Host

Rabbit

Species Reactivity

Human

Isotype

IgG

Cellular Localization

Nucleus

Gene Name

LEF1

Mol. Weight of Antigen

44kDa

Immunogen

Recombinant fragment (around aa100-200) of human LEF1 protein (exact sequence is proprietary)

Database Links

Entrez Gene ID

51176

Unigene

726506

SwissProt

Q9UJU2

Additional Information

Clone

LEF1/341R

Human Gene Symbol

LEF1

Chromosome Location

4q25

Known Applications & Suggested Dilutions

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

PubMed Links

Storage & Stability

Antibody with azide store at 2 to 8 °C. Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.