Printable Datasheet

Recombinant IDH1 (Isocitrate Dehydrogenase) Antibody [IDH1.R132H/7328R]

In Stock
Catalog Number Formulation Size Price
3417-RBM8-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
3417-RBM8-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
3417-RBM8-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant IDH1 (Isocitrate Dehydrogenase) Antibody

FFPE human prostate with IDH1-R132H mutation stained with IDH1-R132H Recombinant Rabbit MAb (IDH1.R132H/7328R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Recombinant IDH1 (Isocitrate Dehydrogenase) Antibody - Image 2

FFPE human brain with IDH1-R132H mutation stained with IDH1-R132H Recombinant Rabbit MAb (IDH1.R132H/7328R). Inset: PBS instead of primary antibody; secondary only negative control.

IDH1 Antibody in SDS-PAGE

IDH1 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Isocitrate dehydrogenase [NADP] cytoplasmic Recombinant Rabbit Monoclonal Antibody (IDH1.R132H/7328R). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

IDH1 R132H antibody binds to IDH1-mutated protein, but does not bind the wild-type IDH1 protein. IDH1 R132H point mutations are frequently seen in World Health Organization grade II and III gliomas and are believed to constitute an early step in tumorigenesis. IDH1 R132H can be used as a diagnostic marker to help differentiate infiltrating gliomas from gliosis, and as a prognostic marker for gliomas and secondary glioblastoma multiforme. IDH1 R132H antibody shows strong cytoplasmic staining and weaker nuclear staining in tumor cells with the R132H-mutated peptide. Diffuse staining of the fibrillary tumor matrix is also seen.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cytoplasm, Cytosol, Peroxisome
Gene Name
IDH1
Positive Control
Human breast, colon or prostate carcinoma. HeLa, HepG2, HT29 or MCF7 cells.
Immunogen
Recombinant fragment (around aa126-137) of the human IDH1-R132H protein (exact sequence is proprietary)
Alternate Names
Isocitrate dehydrogenase [NADP] cytoplasmic, HEL-216|HEL-S-26|IDCD|IDH|IDP|IDPC|PICD

Database Links

Entrez Gene ID
3417
SwissProt
O75874

Additional Information

Clone
IDH1.R132H/7328R
Chromosome Location
2q34
Mol. Weight of Antigen
46.66kDa
Expression System
HEK293 Cells
Purification
Protein A

Related Antibodies

View All IDH1 Antibodies

Functions

  • Catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase (PubMed:10521434, PubMed:19935646). Plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways (PubMed:10521434). May act as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency (By similarity).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
PARTNERSHIP OPPORTUNITIES

NeoBiotechnologies holds Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK
Call Us Now: 1-866-2-NEOBIO (1-866-263-6246)
NeoBiotechnologies Logo
Company Info
Antibodies
Antibody Validation
Research Areas
Resources
Newsletter Signup Updates & Sales