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Formalin-fixed, paraffin-embedded human prostate cancer stained with IDH1-R132G Recombinant Rabbit Monoclonal Antibody (IDH1.R132G/13054R). Inset: PBS instead of primary antibody; secondary only negative control.
SDS-PAGE Analysis of Purified IDH1-R132G Recombinant Rabbit Monoclonal Antibody (IDH1.R132G/13054R). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of human Kidney tissue lysate using IDH1-R132G Recombinant Rabbit Monoclonal Antibody (IDH1.R132G/13054R).
Western Blot Analysis of human liver tissue lysate using IDH1-R132G Recombinant Rabbit Monoclonal Antibody (IDH1.R132G/13054R).
Western Blot Analysis of liver tissue lysates of different species using IDH1-R132G Recombinant Rabbit Monoclonal Antibody (IDH1.R132G/13054R).
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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The gene represented in this entry is involved in disease pathogenesis. Mutations affecting Arg-132 are tissue-specific, and suggest that this residue plays a unique role in the development of high-grade gliomas. Mutations of Arg-132 to Cys, His, Leu or Ser abolish magnesium binding and abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate. Elevated levels of R(-)-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors.
Genetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma. Mutations of Arg-132 to Cys, Gly or His abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate.
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